Effect of radiotherapy on the expression of DNA methyltransferase in cervical cancer

  • XU Han ,
  • GAO Yang ,
  • BAI Jing ,
  • CAI Zhiping ,
  • SHAO Guo
Expand
  • 1. School of Basic Medicine and Forensic Medicine, Baotou Medical College, Inner Mongolia University of Scienceand Technology, Baotou 014040, China;
    2. Inner Mongolia Key Laboratory of Hypoxic Adaptation and Translational Medicine, Baotou Medical College, Inner Mongolia University of Science and Technology;
    3. Department of Respiratory, The Second Affiliated Hospital of Baotou Medical College;
    4. Department of radiotherapy,Baotou Tumor Hospital;
    5. Translational Medicine Center, The Third People’s Hospital of Longgang District of Shenzhen city

Received date: 2023-02-07

  Online published: 2024-01-09

Abstract

Objective: To investigate the effect of radiotherapy on the expression of DNA methyltransferase (DNMT) 1, 3A, and 3B in cervical cancer (CIN) tissues, and to explore its role in radiotherapy resistance. Methods: The cervical cancer tissues of 12 patients were selected before, during and after radiotherapy. The mRNA expression levels of DNMT1, DNMT3A and DNMT3B in cervical cancer tissues after radiotherapy were detected by qPCR. Results: The relative expression levels of DNMT1 to β-ACTIN were 0.122 9±0.021 7, 0.569 6±0.021 7 and 0.162 0±0.035 2 respectively before, during and after radiotherapy for cervical cancer. The expression of DNMT1 mRNA in cervical cancer tissues during and before radiotherapy was significantly different (P<0.05), and the expression of DNMT1 mRNA in cervical cancer tissues during and after radiotherapy was significantly different (P<0.05). The expression of DNMT1 mRNA in cervical cancer tissues before and after radiotherapy was significantly different (P<0.05). The relative expression levels of DNMT3A to β-ACTIN mRNA were 0.001 2±0.000 1, 0.002 1±0.000 1 and 0.001 4±0.000 1 respectively. There were significant differences in DNMT3A mRNA expression in cervical cancer tissues during radiotherapy and before radiotherapy (P<0.05). The expression of DNMT3A mRNA in cervical cancer tissues during and after radiotherapy was significantly different (P<0.05), however, the expression of DNMT3A mRNA before and after radiotherapy was not significantly different (P>0.05). The relative expression of DNMT3B on β-ACTIN mRNA was 0.662 7±0.034 8, 0.810 4±0.084 5 and 0.239 1±0.047 0 respectively. The mRNA expression of DNMT3B was significantly different in cervical cancer tissues during and before radiotherapy (P<0.05). The expression of DNMT3B mRNA in cervical cancer tissues during and after radiotherapy was significantly different (P<0.05), and the expression of DNMT3B mRNA in cervical cancer tissues before and after radiotherapy was significantly different (P<0.05). Conclusion: Radiotherapy changes the expression of DNMT1, DNMT3A and DNMT3B in cervical cancer tissues, which may play an important role in radioresistance.

Cite this article

XU Han , GAO Yang , BAI Jing , CAI Zhiping , SHAO Guo . Effect of radiotherapy on the expression of DNA methyltransferase in cervical cancer[J]. Journal of Baotou Medical College, 2024 , 40(1) : 8 -12 . DOI: 10.16833/j.cnki.jbmc.2024.01.002

References

[1] Alghamian Y, Alchamat GA, Murad H, et al. Effects of γ-radiation on cell growth, cell cycle and promoter methylation of 22 cell cycle genes in the 1321NI astrocytoma cell line[J]. Advn Med Sci, 2017, 62(2): 330-337.
[2] Chi HC, Tsai CY, Tsai MM, et al. Impact of DNA and RNA methylation on radiobiology and cancer progression[J]. Int J Mol Sci, 2018, 19(2): 555.
[3] 白静, 刘娜, 丁力, 等. 放射治疗后宫颈癌组织内差异表达基因鉴定[J]. 基础医学与临床, 2022, 42(2): 215-220.
[4] Barney BM, Petersen IA, Dowdy SC, et al. Intraoperative electron beam radiotherapy (IOERT) in the management of locally advanced or recurrent cervical cancer[J]. Radiat Oncol, 2013, 8(1): 1-9.
[5] Chen Y, Zhao Y, Yang XJ, et al. USP44 regulates irradiation-induced DNA double-strand break repair and suppresses tumorigenesis in nasopharyngeal carcinoma[J]. Nat Commun, 2022, 13(1): 501.
[6] Sakakura C, Miyagawa K, Fukuda KI, et al. Frequent silencing of RUNX3 in esophageal squamous cell carcinomas is associated with radioresistance and poor prognosis[J]. Oncogene, 2007, 26(40): 5927-5938.
[7] Olson RA, Brastianos PK, Palma DA. Prognostic and predictive value of epigenetic silencing of MGMT in patients with high grade gliomas: a systematic review and meta-analysis[J]. J Neuroncol, 2011, 105(2): 325-335.
[8] Liu SS, Leung RC, Chan KY, et al. p73 expression is associated with the cellular radiosensitivity in cervical cancer after radiotherapy[J]. Clin Cancer Res, 2004, 10(10): 3309-3316.
[9] Wu NYY, Zhang XY, Zhu MC, et al. ZNF582 promoter methylation predicts cervical cancer radiosensitivity and ZNF582 protein overexpression reduces radiosensitivity by cell cycle arrest in S phase[J]. Epigenetics, 2022, 17(12): 1786-1799.
[10] Liu S, Pan XF, Yang Q, et al. MicroRNA-18a enhances the radiosensitivity of cervical cancer cells by promoting radiation-induced apoptosis[J]. Oncol Rep, 2015, 33(6): 2853-2862.
[11] Deng Q, Huang CM, Chen N, et al. Chemotherapy and radiotherapy downregulate the activity and expression of dna methyltransferase and enhance Bcl-2/E1B-19-KDa interacting protein-3-induced apoptosis in human colorectal cancer cells[J]. Chemotherapy, 2012, 58(6): 445-453.
[12] Wu C, Guo EG, Ming J, et al. Radiation-induced DNMT3B promotes radioresistance in nasopharyngeal carcinoma through methylation of P53 and P21[J]. Mol Ther Oncolytics, 2020, 17: 306-319.
[13] Liu ZJ, Lin HM, Gan Y, et al. P16 methylation leads to paclitaxel resistance of advanced non-small cell lung cancer[J]. J Cancer, 2019, 10(7): 1726-1733.
[14] Zhang SM, Wang WQ, Wu X, et al. MiR-16-5p modulates the radiosensitivity of cervical cancer cells via regulating coactivator-associated arginine methyltransferase 1[J]. Pathol Int, 2020, 70(1): 12-20.
[15] Li Q, Wei X, Zhou ZW, et al. GADD45α sensitizes cervical cancer cells to radiotherapy via increasing cytoplasmic APE1 level[J]. Cell Death Dis, 2018, 9(5): 524.
[16] Jiao XL, Zhang SY, Jiao J, et al. Promoter methylation of SEPT9 as a potential biomarker for early detection of cervical cancer and its overexpression predicts radioresistance[J]. Clin Epigenetics, 2019, 11(1): 120.
[17] Wang SM, Zhang R, Claret FX, et al. Involvement of microrna-24 and DNA methylation in resistance of nasopharyngeal carcinoma to ionizing radiation[J]. Mol Cancer Ther, 2014, 13(12): 3163-3174.
[18] Kuhmann C, Weichenhan D, Rehli M, et al. DNA methylation changes in cells regrowing after fractioned ionizing radiation[J]. Radiother Oncol, 2011, 101(1): 116-121.
[19] Jiang W, Li YQ, Liu N, et al. 5-Azacytidine enhances the radiosensitivity of CNE2 and SUNE1 cells in vitro and in vivo possibly by altering DNA methylation[J]. PLoS One, 2014, 9(4): e93273.
Outlines

/