Objective: To investigate the effect of radiotherapy on the expression of DNA methyltransferase (DNMT) 1, 3A, and 3B in cervical cancer (CIN) tissues, and to explore its role in radiotherapy resistance. Methods: The cervical cancer tissues of 12 patients were selected before, during and after radiotherapy. The mRNA expression levels of DNMT1, DNMT3A and DNMT3B in cervical cancer tissues after radiotherapy were detected by qPCR. Results: The relative expression levels of DNMT1 to β-ACTIN were 0.122 9±0.021 7, 0.569 6±0.021 7 and 0.162 0±0.035 2 respectively before, during and after radiotherapy for cervical cancer. The expression of DNMT1 mRNA in cervical cancer tissues during and before radiotherapy was significantly different (P＜0.05), and the expression of DNMT1 mRNA in cervical cancer tissues during and after radiotherapy was significantly different (P＜0.05). The expression of DNMT1 mRNA in cervical cancer tissues before and after radiotherapy was significantly different (P＜0.05). The relative expression levels of DNMT3A to β-ACTIN mRNA were 0.001 2±0.000 1, 0.002 1±0.000 1 and 0.001 4±0.000 1 respectively. There were significant differences in DNMT3A mRNA expression in cervical cancer tissues during radiotherapy and before radiotherapy (P＜0.05). The expression of DNMT3A mRNA in cervical cancer tissues during and after radiotherapy was significantly different (P＜0.05), however, the expression of DNMT3A mRNA before and after radiotherapy was not significantly different (P＞0.05). The relative expression of DNMT3B on β-ACTIN mRNA was 0.662 7±0.034 8, 0.810 4±0.084 5 and 0.239 1±0.047 0 respectively. The mRNA expression of DNMT3B was significantly different in cervical cancer tissues during and before radiotherapy (P＜0.05). The expression of DNMT3B mRNA in cervical cancer tissues during and after radiotherapy was significantly different (P＜0.05), and the expression of DNMT3B mRNA in cervical cancer tissues before and after radiotherapy was significantly different (P＜0.05). Conclusion: Radiotherapy changes the expression of DNMT1, DNMT3A and DNMT3B in cervical cancer tissues, which may play an important role in radioresistance.
XU Han
,
GAO Yang
,
BAI Jing
,
CAI Zhiping
,
SHAO Guo
. Effect of radiotherapy on the expression of DNA methyltransferase in cervical cancer[J]. Journal of Baotou Medical College, 2024
, 40(1)
: 8
-12
.
DOI: 10.16833/j.cnki.jbmc.2024.01.002
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