Objective: To analyze the changes of metabolites in serum of children with Mycoplasma pneumoniae pneumonia by metabolomics, in order to screen out potential biomarkers related to Mycoplasma pneumoniae pneumonia and analyze related pathways. Methods: Children with mycoplasma pneumoniae pneumonia admitted to the Department of Pediatrics, the Affliated Hospital of Inner Mongolia Medical University from May 2022 to July 2022 were selected as the case group, and age-matched healthy children were selected as the control group. A total of 2 mL fasting venous blood was collected from the subjects in the morning. UPLC-Q-TOF-MS method was used to analyze the metabolites in serum, and pathway enrichment analysis was performed. Results: A total of 243 metabolites were screened by positive and negative ion models, and 19 metabolites were different. Compared with the control group, 6 substances increased and 13 substances decreased in the case group. Pathway enrichment analysis showed that metabolic pathways included amino acid biosynthesis, cholesterol metabolism and sphingolipid signaling pathway. Conclusion: The serum non-targeted metabolome of children with Mycoplasma pneumoniae pneumonia is different from that of normal children, and phosphatidylcholine has some predictive value for Mycoplasma pneumoniae pneumonia.
WANG Yanfang
,
ZHAO Jijun
,
WANG Yafei
,
WU Hairong
. Analysis of serum biomarkers in children with Mycoplasma pneumoniae pneumonia based on histological techniques[J]. Journal of Baotou Medical College, 2023
, 39(12)
: 40
-44
.
DOI: 10.16833/j.cnki.jbmc.2023.12.008
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