Objective: To investigate the effects of total glycosides of cistanche (GCs) on oxidative stress injury and neuronal apoptosis in rats with cerebral ischemia-reperfusion injury (CIRI) and its possible mechanism. Methods: The rat model of middle cerebral artery occlusion (MCAO) was made by thread embolism. After successful modeling, rats in the drug group (GCs) were given GCs 50 mg/(kg·d) for 14 days; rats in the blank group (Sham) and model group (Model) were gavaged with the same volume of normal saline. After the end of administration, Longa score was used to evaluate the neurological deficit. Subsequently, the rats were sacrificed, and the percentage of cerebral infarction area was calculated by TTC staining. The content of malondialdehyde (MDA), the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in serum were detected by ELISA. Western blot was used to detect the expression of nuclear factor E2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), B-cell lymphoma/leukemia-2 (Bcl-2 ), Bcl-2-related X protein (Bax) and cysteine aspartic protease-3 (Caspase-3) in brain tissue. Results: Compared with the Sham group, the neurological deficit score, the percentage of cerebral infarction area, MDA content, Bax and Caspase-3 protein expression levels in the Model group were increased, while SOD and GSH-Px activities,Bcl-2,Nrf 2,and HO-1 protein expression levels in the Model group were decreased; compared with the Model group, the neurological deficit score, the percentage of cerebral infarction area, MDA content, Bax and Caspase-3 protein expression levels in GCs group were decreased, while SOD and GSH-Px activities, Bcl-2, Nrf 2, and HO-1 protein expression levels in GCs group were increased. Conclusion: GCs can effectively reduce cerebral ischemia-reperfusion injury, which may be related to activating Nrf 2/HO-1 pathway, reducing oxidative stress levels, and inhibiting apoptosis of nerve cells.
YUAN Yuting
,
JIA Jianxin
,
YANG Zhifu
. Effects of total glycosides of cistanche on oxidative stress injury and apoptosis in rats with cerebral ischemia-reperfusion[J]. Journal of Baotou Medical College, 2023
, 39(12)
: 8
-12
.
DOI: 10.16833/j.cnki.jbmc.2023.12.002
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