Objective: To study the mechanism of total flavonoids of Dracocephalummoldavica L. (TFD) on improving nerve injury in Parkinson's disease model mice. Methods: A total of 120 mice were randomly divided into normal control group (n=20) and observation group (n=100). The mice in the observation group were intraperitoneally injected with 30 mg/(kg·d) PTP to prepare PD mouse model, and the mice in the control group were intraperitoneally injected with the same volume of normal saline. After 7 days, the mice in the observation group were randomly divided into 5 groups : model group and TFD treatment group [25, 50, 75, 100 mg/(kg·d )]. The mice in the treatment group were given intragastric administration according to the body weight of the mice, and the mice in the normal control group and the model group were given an equal volume of normal saline for 1 month. The motor function of mice was evaluated by behavioral experiments. The number of positive staining cells of tyrosine hydroxylase (TH) and selective autophagy adaptor protein (P62) in the substantia nigra of mice was detected by immunofluorescence histochemistry. The expression levels of microtubule-associated protein 1 light chain 3β (LC3B), P62, autophagy effector protein (Beclin1) and TH mRNA and protein in the substantia nigra of mice were detected by fluorescence quantitative PCR and Western-blot. Results: TFD treatment significantly increased the suspension motion score, movement distance and fall latency of PD model mice (P＜0.05). TFD treatment increased the number of positive staining cells expressing P62 and TH in the substantia nigra of PD model mice, and TFD treatment increased the number of positive staining cells expressing PINK1, Parkin, P62 and TH proteins in the substantia nigra of PD model mice (P＜0.05). Conclusion: TFD may improve autophagy by up-regulating LC3B, P62 and Beclin1, and promote the clearance of damaged mitochondria and other organelle of dopaminergic neurons, thus producing neuroprotective effects on MPTP-induced PD model mice.