Objective: To investigate the protective effect and possible mechanisms of 1, 25-dihydroxy vitamin D3 on kidney in rats with type 2 diabetes mellitus. Methods: Sprague-Dawley male rats(n=40)were used in this experiments. All rats were randomly divided into the control group(n=8),model group(n=8), low-dose vitamin D3 group(n=8),high-dose vitamin D3 group(n=8), acarbose treatment group(n=8). Intragastric administration were continuously performed on all rats for 6 weeks and then were killed. The serum creatinine (Scr) and urea nitrogen (BUN) levels of rats were measured. The levels of superoxide dismutase (SOD), catalase (CAT), total nitric oxide synthase (tNOS) and hydrogen peroxide (H2O2) of renal tissues were detected. The expression of PI3K/Akt pathway related proteins in renal tissues was detected using Western blot. Results: Compared with the normal group, the contents of serum Scr and BUN in model group were significantly increased (P＜0.05). The contents of serum Scr and BUN of rats in groups with different doses of vitamin D3 decreased significantly comparing with the model group (P＜0.05). The contents of H2O2 and tNOS in renal tissue in the model group increased significantly, while the levels of SOD and CAT decreased significantly comparing with the normal group (P＜0.05). Compared with the model group, all the vitamin D3 groups could inhibit the production of H2O2 and tNOS and significantly increase the content of SOD and CAT in renal tissue (P＜0.05). Compared with the normal group, thep-PI3K/PI3K and p-Akt/Akt ration in the model group increased significantly (P＜0.05), but decreased significantly in vitamin D3 groups (P＜0.05). Conclusion: 1, 25-dihydroxy vitamin D3 has protective effect on kidney injury in type 2 diabetes rats, and the mechanism may be related to the regulation of oxidative stress through mediating PI3K / Akt signaling pathway.
XUAN Jiali
. Protective effects of 1, 25-dihydroxy vitamin D3 on kidney in rat models of Type 2 Diabetes Mellitus[J]. Journal of Baotou Medical College, 2023
, 39(11)
: 23
-26
.
DOI: 10.16833/j.cnki.jbmc.2023.11.004
[1] Mcintyre HD, Catalano P, Zhang CL, et al. Gestational diabetes mellitus[J]. Nat Rev Dis Primers, 2019, 5(1):47.
[2] Wang MM, Chen ZJ, Hu Y, et al. The effects of vitamin D supplementation on glycemic control and maternal-neonatal outcomes in women with established gestational diabetes mellitus:a systematic review and meta-analysis[J]. Clin Nutr, 2021, 40(5):3148-3157.
[3] Li JD, Wang TQ, Liu PP, et al. Hesperetin ameliorates hepatic oxidative stress and inflammation via the PI3K/AKT-Nrf2-ARE pathway in oleic acid-induced HepG2 cells and a rat model of high-fat diet-induced NAFLD[J]. Food Funct, 2021, 12(9):3898-3918.
[4] Feng B, Dong ZL, Wang YR, et al. Li-ESWT treatment reduces inflammation, oxidative stress, and pain via the PI3K/AKT/FOXO1 pathway in autoimmune prostatitis rat models[J]. Andrology, 2021, 9(5):1593-1602.
[5] 石杰, 高艳均, 王倩. 2型糖尿病患者糖尿病肾病患病率及其危险因素分析[J]. 华南预防医学, 2021, 47(2):228-231.
[6] 高娇, 张静, 董瑾, 等. FPG、HbA1c、Sur、Scr、CysC、C1q对早期糖尿病肾病的诊断价值[J]. 标记免疫分析与临床, 2022, 29(7):1093-1097.
[7] Chen YY, Tang JC, Zhang YH, et al. Astaxanthin alleviates gestational diabetes mellitus in mice through suppression of oxidative stress[J]. Naunyn Schmiedebergs Arch Pharmacol, 2020, 393(12):2517-2527.
[8] Rehman K, Akash MSH. Mechanism of generation of oxidative stress and pathophysiology of type 2 diabetes mellitus:how are they interlinked[J]. J Cell Biochem, 2017, 118(11):3577-3585.
[9] Kooshki F, Tutunchi H, Vajdi M, et al. A comprehensive insight into the effect of chromium supplementation on oxidative stress indices in diabetes mellitus:a systematic review[J]. Clin Exp Pharmacol Physiol, 2021, 48(3):291-309.
[10] Chen SH, Liu XN, Peng Y. MicroRNA-351 eases insulin resistance and liver gluconeogenesis via the PI3K/AKT pathway by inhibiting FLOT2 in mice of gestational diabetes mellitus[J]. J Cell Mol Med, 2019, 23(9):5895-5906.
[11] 吴浩, 符丽珍, 赵勇, 等. 桔梗皂苷D通过介导PI3K/Akt/mTOR信号通路调节氧化应激改善糖尿病肾病模型大鼠肾损伤[J]. 中国药理学与毒理学杂志, 2022, 36(3):170-176.
[12] Zong HY, Wang EL, Han YM, et al. Effect of miR-29b on rats with gestational diabetes mellitus by targeting PI3K/Akt signal[J]. Eur Rev Med Pharmacol Sci, 2019, 23(6):2325-2331.
