Objective: To clarify whether general cistanosides (GCs) ameliorates cognitive dysfunction in rapidly aging mice (SAMP8) by mediating specific androgen receptor (AR) in regulation of expression of synaptic protein. Methods: A total of 76 7-month-old male SAMP8 mice were randomly divided into the model group, GCs group, GCs + flutamide (F) group, GCs + fulvestrant (ICI) group, F group, and ICI group. The learning and memory functions of mice in each group were tested with Morris water maze. Western blotting and RT-PCR were used to test the expression level of synaptophysin (SYN), postsynaptic density-95 (PSD-95) and the brain-derived neurotrophic factor (BDNF) of mice in each group. Results: GCs significantly improved the learning and cognitive function of SAMP8 mice. Comparing with the GCs group, the learning and memory function of mice in the GCs+F group and GCs+ICI group was significantly lowered. Western blotting and RT-PCR results showed that the expression level of BDNF, SYN, and PSD-95 were significantly increased at the protein level and gene level in the GCs group compared with the Model group (P＜0.05). SYN expression level was increased in the GCs+F and GCs+ICI group, and expression levels of BDNF and PSD-95 were significantly decreased in the GCs+F and GCs+ICI group compared with the GCs group (P＜0.05). Conclusion: GCs may specifically mediate AR to enhance synaptic plasticity in SAMP8 mice, which in turn improve learning and cognitive function.
WU Xiaorong
,
ZHU Shilong
,
GONG Shiji
,
YANG Zhanjun
,
JIA Jianxin
. Effects of general cistanosides mediated specific androgen receptors on synaptic proteins and cognitive function in SAMP8 mice[J]. Journal of Baotou Medical College, 2023
, 39(11)
: 1
-5
.
DOI: 10.16833/j.cnki.jbmc.2023.11.001
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