Objective: To clarify whether general cistanosides (GCs) ameliorates cognitive dysfunction in rapidly aging mice (SAMP8) by mediating specific androgen receptor (AR) in regulation of expression of synaptic protein. Methods: A total of 76 7-month-old male SAMP8 mice were randomly divided into the model group, GCs group, GCs + flutamide (F) group, GCs + fulvestrant (ICI) group, F group, and ICI group. The learning and memory functions of mice in each group were tested with Morris water maze. Western blotting and RT-PCR were used to test the expression level of synaptophysin (SYN), postsynaptic density-95 (PSD-95) and the brain-derived neurotrophic factor (BDNF) of mice in each group. Results: GCs significantly improved the learning and cognitive function of SAMP8 mice. Comparing with the GCs group, the learning and memory function of mice in the GCs+F group and GCs+ICI group was significantly lowered. Western blotting and RT-PCR results showed that the expression level of BDNF, SYN, and PSD-95 were significantly increased at the protein level and gene level in the GCs group compared with the Model group (P<0.05). SYN expression level was increased in the GCs+F and GCs+ICI group, and expression levels of BDNF and PSD-95 were significantly decreased in the GCs+F and GCs+ICI group compared with the GCs group (P<0.05). Conclusion: GCs may specifically mediate AR to enhance synaptic plasticity in SAMP8 mice, which in turn improve learning and cognitive function.
WU Xiaorong
,
ZHU Shilong
,
GONG Shiji
,
YANG Zhanjun
,
JIA Jianxin
. Effects of general cistanosides mediated specific androgen receptors on synaptic proteins and cognitive function in SAMP8 mice[J]. Journal of Baotou Medical College, 2023
, 39(11)
: 1
-5
.
DOI: 10.16833/j.cnki.jbmc.2023.11.001
[1] Soria Lopez JA, González HM, Léger GC. Alzheimer's disease [M]//Handbook of Clinical Neurology. Amsterdam: Elsevier, 2019: 231-255.
[2] Lašaitè L, Ceponis J, Preikša RT, et al. Effects of two-year testosterone replacement therapy on cognition, emotions and quality of life in young and middle-aged hypogonadal men[J]. Andrologia, 2017, 49(3): e12633.
[3] Jia JX, Yan XS, Song W, et al. The protective mechanism underlying phenylethanoid glycosides (PHG) actions on synaptic plasticity in rat Alzheimer's disease model induced by beta amyloid 1-42[J]. J Toxicol Environ Health A, 2018, 81(21): 1098-1107.
[4] 刘心朗, 周丽丽, 杨占君, 等. 肉苁蓉总苷对SAMP8小鼠学习记忆能力及突触可塑性的影响[J]. 中华中医药学刊, 2022, 40(1): 110-115,276-277.
[5] 宋嵬, 白雨朦, 李晓宇, 等. 睾酮对β-淀粉样蛋白1-42寡聚体联合去势大鼠海马内突触素表达的影响[J]. 解剖学杂志, 2019, 42(6): 547-550.
[6] Chen L, Huang ZL, Du YH, et al. Capsaicin attenuates amyloid-β-induced synapse loss and cognitive impairments in mice[J]. J Alzheimers Dis, 2017, 59(2): 683-694.
[7] Kische H, Gross S, Wallaschofski H, et al. Associations of androgens with depressive symptoms and cognitive status in the general population[J]. PLoS One, 2017, 12(5): e0177272.
[8] 王璐, 白雨朦, 李晓宇, 等. 肉苁蓉总苷对阿尔茨海默病模型大鼠学习认知功能和氧化应激的影响[J]. 解剖学杂志, 2020, 43(3): 194-199, 275.
[9] Nguyen VT, Hill B, Sims N, et al. Brain-derived neurotrophic factor rs6265 (Val66Met) single nucleotide polymorphism as a master modifier of human pathophysiology[J]. Neural Regen Res, 2023, 18(1): 102-106.
[10] Cousin MA. Synaptophysin-dependent synaptobrevin-2 trafficking at the presynapse-mechanism and function[J]. J Neurochem, 2021, 159(1): 78-89.
[11] Liang HZ, Wang HQ, Wang SS, et al. 3D imaging of PSD-95 in the mouse brain using the advanced CUBIC method[J]. Mol Brain, 2018, 11(1): 50.