Objective: To investigate the value of circulating cell-free DNA (cfDNA) in the early diagnosis and disease activity of patients with rheumatoid arthritis (RA). To explore the relationship between cfDNA content and immunological diagnostic indicators of RA and related indicators of disease activity evaluation (rheumatoid factor (RF), Anti-cyclic citrullinated peptide antibodies (anti-CCP antibodies), C-reactive protein (CRP), Erythrocyte sedimentation rate (ESR)) and Disease Activity Score of 28 joints (DAS28-ESR). Methods: A total of 80 newly diagnosed RA patients who did not receive anti-rheumatic drugs were selected as the observation group (RA group), and 40 healthy people were selected as the control group (HC group). The serum cfDNA content of two groups was measured and the integrity was calculated. The correlation between cfDNA content and clinical indicators of RA patients was analyzed. Results: The RA group had a significantly higher level of cfDNA than the HC group (P＜0.05), but there was no correlation between and diagnostic serological markers (P＞0.05). According to the disease activity index of 28 joints (DAS28-ESR score), RA patients were stratified by disease activity, and it was found that the cfDNA content in the high DAS28-ESR score group was higher than that in the low DAS28-ESR score group (P＜0.05). cfDNA was correlated with DAS28-ESR, CRP and ESR (P＜0.05). Conclusion: The content of serum cfDNA in RA patients is increased, which is correlated with inflammatory response. It has diagnostic value for RA and may be an independent risk factor for the pathogenesis of RA.
TANG Na
,
ZHANG Wenlan
,
BAI Li
,
PANG Chunyan
,
WANG Yongfu
,
ZHANG Wei
,
HU Tongping
. Relationship between serum cfDNA and clinical indicators in patients with rheumatoid arthritis[J]. Journal of Baotou Medical College, 2023
, 39(10)
: 18
-22
.
DOI: 10.16833/j.cnki.jbmc.2023.10.004
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