Relationship between serum cfDNA and clinical indicators in patients with rheumatoid arthritis

  • TANG Na ,
  • ZHANG Wenlan ,
  • BAI Li ,
  • PANG Chunyan ,
  • WANG Yongfu ,
  • ZHANG Wei ,
  • HU Tongping
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  • 1. Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou 014040, China;
    2. Central Laboratory, the First Affiliated Hospital of Baotou Medical College;
    3. Department of Clinical Laboratory, the First Affiliated Hospital of Baotou Medical College

Received date: 2023-04-18

  Online published: 2023-10-25

Abstract

Objective: To investigate the value of circulating cell-free DNA (cfDNA) in the early diagnosis and disease activity of patients with rheumatoid arthritis (RA). To explore the relationship between cfDNA content and immunological diagnostic indicators of RA and related indicators of disease activity evaluation (rheumatoid factor (RF), Anti-cyclic citrullinated peptide antibodies (anti-CCP antibodies), C-reactive protein (CRP), Erythrocyte sedimentation rate (ESR)) and Disease Activity Score of 28 joints (DAS28-ESR). Methods: A total of 80 newly diagnosed RA patients who did not receive anti-rheumatic drugs were selected as the observation group (RA group), and 40 healthy people were selected as the control group (HC group). The serum cfDNA content of two groups was measured and the integrity was calculated. The correlation between cfDNA content and clinical indicators of RA patients was analyzed. Results: The RA group had a significantly higher level of cfDNA than the HC group (P<0.05), but there was no correlation between and diagnostic serological markers (P>0.05). According to the disease activity index of 28 joints (DAS28-ESR score), RA patients were stratified by disease activity, and it was found that the cfDNA content in the high DAS28-ESR score group was higher than that in the low DAS28-ESR score group (P<0.05). cfDNA was correlated with DAS28-ESR, CRP and ESR (P<0.05). Conclusion: The content of serum cfDNA in RA patients is increased, which is correlated with inflammatory response. It has diagnostic value for RA and may be an independent risk factor for the pathogenesis of RA.

Cite this article

TANG Na , ZHANG Wenlan , BAI Li , PANG Chunyan , WANG Yongfu , ZHANG Wei , HU Tongping . Relationship between serum cfDNA and clinical indicators in patients with rheumatoid arthritis[J]. Journal of Baotou Medical College, 2023 , 39(10) : 18 -22 . DOI: 10.16833/j.cnki.jbmc.2023.10.004

References

[1] Lin YJ, Anzaghe M, Schulke S. Update on the pathomechanism, diagnosis, and treatment options for rheumatoid arthritis[J]. Cells, 2020,9(4):880.
[2] Sparks JA. Rheumatoid Arthritis[J]. Ann Intern Med, 2019,170(1):ITC1-ITC16.
[3] Cush JJ. Rheumatoid arthritis: early diagnosis and treatment[J]. Med Clin North Am, 2021,105(2):355-365.
[4] 刘佳君. 类风湿关节炎颈动脉粥样硬化危险因素分析及血清补体C5意义初探[D]. 遵义:遵义医科大学,2022.
[5] Aletaha D, Smolen JS. Diagnosis and management of rheumatoid arthritis: a review[J]. JAMA, 2018,320(13):1360-1372.
[6] Atzeni F, Talotta R, Masala IF, et al. Biomarkers in rheumatoid arthritis[J]. Isr Med Assoc J, 2017,19(8):512-516.
[7] Littlejohn EA, Monrad SU. Early diagnosis and treatment of rheumatoid arthritis[J]. Prim Care, 2018,5(2):237-255.
[8] 刘颖文. 联合检测降钙素和降钙素原在类风湿性关节炎早期诊断中的价值[D]. 重庆:重庆医科大学,2020.
[9] Jang S, Kwon EJ, Lee JJ. Rheumatoid arthritis: pathogenic roles of diverse immune cells[J]. Int J Mol Sci, 2022,23(2):905.
[10] 刘宇晗,胡廷婷,谭立明. 血清14-3-3η蛋白和胶原三螺旋重复蛋白-1及类风湿因子在类风湿关节炎诊断中的价值[J]. 医学研究生学报, 2022,35(10):1081-1085.
[11] Liang HY, Peng B, Dong C, et al. Cationic nanoparticle as an inhibitor of cell-free DNA-induced inflammation[J]. Nat Commun, 2018,9(1):4291.
[12] Kustanovich A, Schwartz R, Peretz T, et al. Life and death of circulating cell-free DNA[J]. Cancer Biol Ther, 2019,20(8):1057-1067.
[13] 阚志文,黄子杰,崔亚云,等. 电化学方法检测血浆游离DNA甲基化水平与甲状腺癌诊断以及相关临床特征的关系[J]. 实用医学杂志, 2021,37(6):792-796.
[14] Duvvuri B, Lood C. Cell-free DNA as a biomarker in autoimmune rheumatic diseases[J]. Front Immunol, 2019,10:502.
[15] Leon S A, Ehrlich G E, Shapiro B, et al. Free DNA in the serum of rheumatoid arthritis patients[J]. J Rheumatol, 1977,4(2):139-143.
[16] Hashimoto T, Yoshida K, Hashiramoto A, et al. Cell-free DNA in rheumatoid arthritis[J]. Int J Mol Sci, 2021,22(16):8941.
[17] Hashimoto T, Yoshida K, Hashimoto N, et al. Circulating cell free DNA: a marker to predict the therapeutic response for biological DMARDs in rheumatoid arthritis[J]. Int J Rheum Dis, 2017,20(6):722-730.
[18] Radu AF, Bungau SG. Management of rheumatoid arthritis: an overview[J]. Cells, 2021,10(11):2857.
[19] Van-Riel PL, Renskers L. The disease activity score (DAS) and the disease activity score using 28 joint counts (DAS28) in the management of rheumatoid arthritis[J]. Clin Exp Rheumatol, 2016,34(5 Suppl 101):S40-S44.
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