Objective: To investigate the relationship between long non-coding RNA (lncRNA) MVIH and cisplatin resistance in gastric cancer cells and its detailed regulatory mechanism. Methods: The cisplatin-resistant cells of gastric cancer were constructed. The proliferation capacity and cisplatin half-inhibitory concentration (IC50) were detected by CCK8 assay, and the resistance index was calculated. Real-time quantitative PCR (qRT-PCR) was used to analyze the expressions of lncRNA MVIH and let-7b-5p in resistant cells. The apoptosis rate was detected by flow cytometry. The expressions of apoptosis-related proteins Bax and Caspase3 were detected by western blotting. lncRNA MVIH distribution in cells and possible regulated miRNAs were analyzed by lncLocator database and lncRNASNP2 database, and dual luciferase reporter gene assay was used for verification. Bioinformatics was used to analyze the expression of let-7b-5p in gastric cancer and adjacent tissues and its effect on prognosis of gastric cancer. Results: The expression of lncRNA MVIH was higher in gastric cancer cisplatin-resistant cells MGC803R than its parental cells MGC803; transfection of siMVIH in MGC803R significantly increased cisplatin-induced apoptosis, significantly upregulated apoptosis-associated proteins Bax and Caspase3, while promoting the cell proliferation. LncRNA MVIH was enriched in the cytoplasm and regulated let-7b-5p, and let-7b-5p was lowly expressed in gastric cancer and positively correlated with gastric cancer prognosis; co-transfection of siMVIH and let-7b-5p inhibitor in MGC803 reversed the regulation of cisplatin sensitivity by lncRNA MVIH. Conclusion: LncRNA MVIH promotes cisplatin resistance in gastric cancer cells by regulating let-7b-5p.
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