Objective: To analyze the participation of apoptosis-related factors in the mechanism of ginsenoside Rg1 promoting functional recovery after intracerebral hemorrhage in rats. Methods: The rats were randomly divided into three groups, while the rats in the control group were injected with 1 μL of normal saline into the inner capsule under stereotaxic localization, and the rats in the model group were injected with 1 μL of type Ⅶ collagenase. The motor function of the rats was scored on 1st, 7th and 14th day after operation. The ginsenoside group was intraperitoneally injected with ginsenoside Rg1 on the next day after modeling. The brain water content of rats was measured on the 7th day after operation, and the expression of Bcl-2 and Bax in brain tissue was observed by immunohistochemistry. Results: In the model group of intracerebral hemorrhage, there were significant cell morphology damage, unclear cell boundaries, and structural displacement and compression of the nerve cells around the inner capsule; On the 1st, 7th and 14th day, the neurological function score of the model group was higher than that of the control group (P＜0.05), and that in the ginsenoside Rg1 group was lower than that in the model group on the 7th and 14th day (P＜0.05); On the 7th day, the brain water content in the model group was higher than that in the control group (P＜0.05), and that in the ginsenoside Rg1 group was lower than that in the model group (P＜0.05); The number of Bax positive neurons around the inner capsule area in the model group and ginsenoside Rg1 group was more than that in the control group (P＜0.05); The number of Bcl-2 positive cells in brain slices of rats in the model group was lower than that in the control group, and that in the ginsenoside Rg1 group was higher than that in the model group and lower than that in the control group (P＜0.05). Conclusion: The injection of ginsenoside Rg1 after intracerebral hemorrhage in rats is beneficial to the recovery of motor function, and the increase of Bcl-2 positive cells in related brain regions.
SU Nan
,
GONG Tao
,
PANG Hai
,
CHEN Yuling
,
LI Liang
,
SHI Suqin
,
CAI Zhiping
. Effect of ginsenoside Rg1 on apoptosis-related factors in the recovery of neurological function in rats with intracerebral hemorrhage[J]. Journal of Baotou Medical College, 2023
, 39(10)
: 6
-10
.
DOI: 10.16833/j.cnki.jbmc.2023.10.002
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