Objective: To elucidate the differentially expressed genes in reflux esophagitis and explore the possible mechanism of mucosal damage in reflux esophagitis, and preliminarily screen and identify the expression changes and effects of long-chain non-coding RNA HOTAIR in reflux esophagitis. Methods: Transcriptome sequencing and bioinformatics analysis were performed on tissue specimens at mucosal erosion site of reflux esophagitis and mucosa smooth esophageal tissue under para-erosion endoscopy. The expression changes of long-chain non-coding RNA HOTAIR in the two types of tissues were determined by real-time fluorescent quantitative PCR, and its relationship with the age of patients with reflux esophagitis was analyzed. Results: The transcriptome sequencing results showed that there were a variety of genes differentially expressed between the erosive group and the para-erosive group of reflux esophagitis, of which 7661 genes were down-regulated. Bioinformatics analysis showed that differentially expressed genes were enriched in immune responses. For the screened long-chain non-coding RNA HOTAIR, real-time fluorescent quantitative PCR showed that its relative expression was correlated with the age of patients with reflux esophagitis (P＜0.05). Compared with the para-erosion group, the expression of HOTAIR was up-regulated in the erosion group of reflux esophagitis in the older age group (P＜0.05), and down-regulated in the erosion group of reflux esophagitis in the younger age group (P＜0.05). Conclusion: The differentially expressed genes may be involved in the pathogenesis of reflux esophagitis through immune response. The screened long-chain non-coding RNA HOTAIR expression showed opposite trends in patients of different ages, suggesting that HOTAIR may play an important role in reflux esophagitis and can be used as an important auxiliary indicator for diagnosis.
NIU Lu
,
MENG Xianmei
,
SHAO Guo
. Preliminary screening and identification of long-chain non-coding RNA HOTAIR related to reflux esophagitis based on transcriptome sequencing[J]. Journal of Baotou Medical College, 2023
, 39(9)
: 34
-39
.
DOI: 10.16833/j.cnki.jbmc.2023.09.007
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