Objective: To study the analgesic effect of gross saponins from tribulus terrestris (GSTT) on peripheral neuropathic pain caused by chronic constriction injury (CCI) in rats based on TLR4/NF-κB pathway. Methods: A total of 40 male SD rats were randomly divided into the SHAM group, CCI group, GSTT100mg/kg+CCI group, GSTT200mg/kg+CCI group and pregabalin +CCI group (positive control), with 8 rats in each group. Except SHAM group, CCI rat models were prepared in other 4 groups, paw withdrawal mechanical threshold (PWMT) and thermal latency were detected after being processed by drug respectively. HE staining was used to detect the pathological changes of spinal cord and dorsal root ganglion. The expression of NF-κB in spinal cord and dorsal root ganglion was detected by immunohistochemistry. Results: Compared with the SHAM group, PWMT and thermal latency of rats in the CCI group were significantly decreased and the differences were statistically significant (P＜0.05). The neurons and glial cells in the posterior horn of the injured spinal cord and the dorsal root ganglion showed deep staining, swelling and atrophy, with concentration and loss of nucleus. The expression level of NF-κB in the posterior horn and dorsal root ganglion of the injured side was significantly increased. Compared with the CCI group, PWMT and thermal latency of rats in GSTT100 mg/kg+CCI group, 200 mg/kg+CCI group and pregabali +CCI group were increased, and the differences were statistically significant (P＜0.05). The above pathological changes were also significantly improved, and NF-κB level in posterior horn of spinal cord and dorsal root ganglion of injured side was decreased. Compared with the GSTT100 mg/kg+CCI group, the above pathological changes were further improved in the 200 mg/kg+CCI group and regabalin +CCI group, PWMT and thermal latency were significantly increased, and the differences were statistically significant (P＜0.05). Meanwhile, NF-κB levels in the posterior horn of the injured side of the spinal cord and dorsal root ganglion were further reduced. Conclusion: GSTT has analgesic effect on peripheral neuropathic pain in CCI rats, which may be related to TLR4/NF-κB pathway.
GUO Wuxia
,
GAO Huricha
,
CHEN Hong
,
LI Xiaoxu
,
GAO Alatansuhe
,
YU Zhijia
,
ZHAO Zhiying
,
ZHANG Ming
. Analgesic effect of gross saponins from tribulus terrestris on peripheral neuropathic pain based on TLR4/NF-κB pathway[J]. Journal of Baotou Medical College, 2023
, 39(9)
: 12
-18
.
DOI: 10.16833/j.cnki.jbmc.2023.09.003
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