Objective: To study the molecular mechanism of hypoxic preconditioning on the protection of hypoxia injury by altering the expression of MALAT1. Methods: A total of 90 ICR mice (18 ~ 22g, 6 ~ 8 weeks) were randomly divided into three groups: Control group (n=30), Hypoxic group (n=30), and Hypoxic preconditioning (HPC) group (n=30). The mRNA level of MALAT1 in the hippocampus of mice was detected by qPCR. MALAT1 was knocked down by siRNA in HT22 cells, and then H/R (Hypoxia/Reoxygenation) injury was performed on HT22 cells. The mRNA level of MALAT1 was detected by QPCR after knockdown of MALAT1 in HT22 cells. The expression changes of Cleaved Caspase-3 and cell death rate after knockdown of MALAT1 in HT22 cells followed by H/R injury were detected by Western blot and live cell workstation. Results: Compared with Group C, the expression of MALAT1 in hypoxia group was significantly increased (P＜0.05), and the expression of MALAT1 in HPC group was recovered (P＜0.05) . Compared with NC Group, the mRNA expression level of MALAT1 in siMALAT1 group decreased (P＜0.05). The expression level of Cleaved Caspase-3 in siMALAT1 + H/R group was significantly higher than that in siMALAT1 and H/R groups (P＜0.05). Compared with H/R group, the cell death rate of siMALAT1 + H/R group was significantly higher (P＜0.05). Conclusion: Hypoxic preconditioning may play a neuroprotective role by changing the expression of MALAT1 and decreasing apoptosis induced by hypoxia.
WANG Liping
,
JIANG Shuyuan
,
SHAO Guo
. Neuroprotective effect of MALAT1 in hypoxic preconditioned mice[J]. Journal of Baotou Medical College, 2023
, 39(8)
: 6
-11
.
DOI: 10.16833/j.cnki.jbmc.2023.08.002
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