Objective: To observe the regenerative effect of Mongolian medicine Eerdun Wurile (EW) on zebrafish heart injury and its possible mechanism, and provide experimental basis for further research on Mongolian medicine Eerdun Wurile. Methods: The animal model of ventricular injury was established using the transgenic line Tg (vmhc: mCherry) treated with metronidazole (MTZ). The zebrafish embryos at 3 dpf(days post fertilization) were randomly divided into the control group (DMSO group,n=30), the experimental myocardial ablation group (MTZ group, n=30) and the Eerdun Wurile treatment group (MTZ+EW, n=30) after ventricular injury. Three groups were treated with 25 ug/ml Eerdun Wurile, a Mongolian medicine, after MTZ treatment for 24 hours. Three groups were tested for cell proliferation at 72 hpt. Cell proliferation were observed with confocal microscope and quantitatively analyzed. At the same time, NOS, iNOS, TNF-α, IL-1β and IL-6 were analyzed with real time qPCR. Results: After the treatment with Mongolian medicine Erden-Uhelle, the proportion of regeneration and repair after myocardial injury was significantly increased in the treatment group. And the proliferation of regenerated myocardial cells was enhanced in Eerdun Wurile treated group. The expression of inflammatory factors was significantly lower than that of the control group. Conclusion: Mongolian medicine Eerdun Wurile can promote the repair and regeneration of zebrafish embryonic cardiac muscle cells, promote the proliferation of cardiac muscle cells, and inhibit the inflammatory reaction after zebrafish embryonic ventricular injury.
LIN Yufeng
,
ZHAO Rui
,
CHEN Xianghui
,
ZHU Wei
,
LI Xiaofeng
,
XIE Yabin
,
XIE Wei
,
BADE Rengui
,
JIANG Shuyuan
,
LIU Xiaolei
,
SHAO Guo
,
BAI Haihua
,
JIA Xiaoe
. Mongolian medicine Eerdun Wurile promotes myocardial cells repair and regeneration through cell proliferation and inflammation inhibition[J]. Journal of Baotou Medical College, 2023
, 39(8)
: 1
-5
.
DOI: 10.16833/j.cnki.jbmc.2023.08.001
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