Objective: To study whether suberoylanilide hydroxamic acid (SAHA ) can regulate RECK protein to change the expression of MMP-2 and MMP-9 in animals, so as to inhibit the growth, proliferation and metastasis of breast cancer cells, improve the prognosis, and provide new ideas for clinical treatment of breast cancer. Methods: The breast cancer cell MCF-7 was cultured and transplanted subcutaneously into nude mice to make an animal model. The nude mouse tumor model was divided into control group ( normal saline 0.4 mL/kg ) and experimental group ( SAHA 0.42 mg/kg). After the tumor model was established, the content of IL-6 in nude mice was detected by ELISA. The nude mice were sacrificed by neck cutting method, and the tumor tissues were weighed and the organ index was calculated. HE pathological staining was performed on tumor tissues. The expression of MMP-2, MMP-9 and RECK in tumor tissues was detected by IHC and WB. Results: Compared with the control group, the IL-6 content, tumor weight and organ index of the experimental group decreased (P＜0.01). HE staining of tumor tissue showed that the experimental group was mainly moderate to severe necrosis, while the control group was mainly mild to moderate necrosis. IHC test showed that compared with the control group, the positive expression of RECK in the experimental group was higher, and the positive expression of MMP-2 and MMP-9 was lower (P＜0.05). WB test showed that compared with the control group, the expression of RECK in the experimental group was higher, and the expression of MMP-2 and MMP-9 was lower (P＜0.01). Conclusion: SAHA can reduce the expression of MMP-2 and MMP-9 by increasing the expression of RECK protein in animals, thus inhibiting the invasion and metastasis of human breast cancer MCF-7 cell.
YU Yicheng
,
JIAO Kailun
,
HE Tao
. Effect of SAHA on human breast cancer cell MCF-7 transplanted tumor in nude mice[J]. Journal of Baotou Medical College, 2023
, 39(6)
: 40
-46
.
DOI: 10.16833/j.cnki.jbmc.2023.06.009
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