Objective: To investigate the effects of bariatric surgery on food intake and body weight in obese rats by regulating Ghrelin. Methods: Ninety male 5-week-old SD rats were randomly divided into normal diet feeding group (n=15) and high fat diet feeding group (n=75). After 5 weeks of high fat diet feeding, the rats in the high fat diet group whose body weight was 20 % higher than that in the normal diet group were considered as successful obesity models. The rats in high-fat diet group were randomly divided into obesity model group, sham operation group and bariatric operation group, with 15 rats in each group. The body weight (once a week) and daily food intake of rats were recorded during the modeling period. The sleeve gastrectomy was performed in the bariatric operation group, and the changes of body mass and food intake in each group were recorded after 1-4 weeks. At the 4th week, blood samples from abdominal aorta were collected to detect the contents of triglyceride (TG) and total cholesterol (TC) in serum, and the protein expression of Ghrelin, mTOR, p-mTOR, p70s6k and p-p70s6k in gastric tissue was detected by WB. Results: Compared with the obesity model group and the sham operation group, the food intake and body weight of obese rats in the bariatric operation group were decreased (P＜0.05). The contents of serum triglyceride and total cholesterol in bariatric surgery group were higher than those in sham operation group and obesity model group at the 4th week after bariatric surgery (P＜0.05). Compared with the obesity model group, the protein expressions of p-mTOR, p-P70S6K and Ghrelin in the gastric tissue of the bariatric surgery group were significantly decreased (P＜0.05). Conclusion: Bariatric surgery may reduce food intake and body weight in obese rats by inhibiting mTOR/ P70S6K pathway and down-regulating the expression of Ghrelin that promotes food intake.
ZHANG Zeyu
,
LI Ruibin
,
WANG Wei
,
WAN Zhiheng
. Effects of bariatric surgery on food intake and body weight of obese rats regulated by Ghrelin[J]. Journal of Baotou Medical College, 2023
, 39(6)
: 30
-34
.
DOI: 10.16833/j.cnki.jbmc.2023.06.007
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