Objective: To observe the effects of curcumin on mitochondrial autophagy and oxidative stress in human renal tubular epithelial cells (HK-2) under high glucose environment, and to explore the protective effect and mechanism of curcumin on HK-2 cells. Methods: HK-2 cells were divided into 4 groups: control group(5.5 mmol/L glucose), curcumin group(5.5 mmol/L glucose +10 μmol/L Cur), high glucose group(30 mmol/L glucose) and curcumin high glucose group(30 mmol/L glucose+10 μmol/L Cur). All groups were stimulated for 48h. Total protein was collected, and the expression of mitochondrial autophagy related proteins Pink1, Parkin, LC3B and beclin1 was detected by Western blot. Mitochondrial membrane potential was detected by JC-1. Reactive oxygen species (ROS) detection kit was used to detect intracellular ROS production. Results: Compared with the control group, the indexes in the curcumin group did not change significantly(P＞0.05). Compared with curcumin group, the protein expressions of Pink1, Parkin, LC3B and Beclin1 in high glucose group were significantly decreased(P＜0.01), intracellular ROS expression increased significantly(P＜0.01), and mitochondrial membrane potential activity decreased(P＜0.01). Compared with high glucose group, the protein expression of Pink1, Parkin, LC3B and Beclin1 in curcumin high glucose group was significantly increased(P＜0.01), intracellular ROS expression was significantly decreased(P＜0.01), mitochondrial membrane potential activity increased(P＜0.01). Conclusion: Curcumin can reverse the mitochondrial autophagy induced by high glucose and inhibit oxidative stress in HK-2 cells.
CHANG Jiang
,
WANG Ying
,
LI Yongyan
,
LAI Lianying
,
YANG Shixu
,
LI Yujie
. Effects of curcumin on mitochondrial autophagy induced by high glucose in human renal tubular epithelial cells[J]. Journal of Baotou Medical College, 2023
, 39(6)
: 24
-29
.
DOI: 10.16833/j.cnki.jbmc.2023.06.006
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