Objective: To investigate the effect and possible mechanism of lung cancer associated transcript 1(LUCAT1) of long non-coding RNA on proliferation, metastasis and cisplatin resistance(DDP) in cervical cancer cells. Methods: The expression of LncLUCAT1 in cancer and pericarcinomatous tissue of 30 patients with cervical cancer was detected by real-time quantitative PCR (qPCR). Cervical cancer SiHa cells were used as experimental cell lines. After siRNA interfering with expression of LncLUCAT1 in SiHa cells, the proliferation ability of cells was detected by MTT and clonogenic assay, and the invasion and migration ability of cells were measured by transwell assay. After LncLUCAT1 being interfered and overexpressed in SiHa/DDP cells, the transfection effect was verified by qPCR, the changes of IC50 were detected by MTT, and the expressions of AKT and p-Akt in SiHa/DDP cells were detected by Western blot. Results: Compared with para-cervical tissues, LncLUCAT1 was highly expressed in cervical cancer tissues. MTT, clonogenic assay and transwell migration assay showed that the proliferation, invasion and migration ability of SiHa cells inhibited by down-regulated LncLUCAT1. qPCR results showed that LncLUCAT1 expression in SiHa/DDP cells was significantly higher than that in SiHa cells (P<0.05). Interference of LncLUCAT1 expression increased the sensitivity of SiHa/DDP to DDP, while overexpression of LncLUCAT1 reduced the sensitivity of SiHa/DDP to DDP. Western blot results showed that interference of LncLUCAT1 expression inhibited p-AKT protein expression, while overexpression of LncLUCAT1 promoted p-AKT protein expression. Conclusion: LncLUCAT1 could regulate the phosphorylation of AKT and reduce the sensitivity of cervical cancer SiHa cells to cisplatin.
WEI Li
,
CHEN Yuzhong
,
DU Jun
,
LI Yan
,
ZHANG Huihui
,
LI Duojie
. Mechanism of LncRNA LUCAT1 promoting proliferation, metastasis and cisplatin resistance in cervical cancer SiHa cells[J]. Journal of Baotou Medical College, 2023
, 39(5)
: 22
-28
.
DOI: 10.16833/j.cnki.jbmc.2023.05.005
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