Regulation of hypoxia inducible factor-1 α on T cell subsets in splenocytes with collagen induced arthritis mice

  • MENG Jin ,
  • KANG Min ,
  • LU Yanrong ,
  • GUO Yu ,
  • PANG Chunyan
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  • 1. Central laboratory, The First Affiliated Hospital of Baotou Medical College (Inner Mongolia Key Laboratory of Autoimmunity), Baotou 014010, China;
    2. Baotou Medical College

Received date: 2022-06-23

  Online published: 2023-05-24

Abstract

Objective: To investigate the regulation of hypoxia inducible factor-1 α( HIF-1α) on T lymphocyte subsets in splenocytes with collagen induced arthritis (CIA) mice by small interfering RNA (siRNA) vector. Methods: the animal model of rheumatoid arthritis was established in DBA / 1 mice were injected by bovine type Ⅱ collagen. CIA mice were injected with HIF-1α-siRNA adenovirus and negative control adenovirus, once a week for 4 weeks. The experiment was divided into control group, model group, negative control group and HIF-1α- siRNA group. The mice were killed after the experiment. The proportion of Th1cells and Th2 cells, Th17 cells and Tregs in mice spleen were detected by flow cytometry, the pathological changes of mouse ankle was observed by HE staining. Results: Compared with control group, the proportion of Th1 cells and Th17 cells in splenocytes of model group increased, the difference was statistically significant (P<0.05), the proportion of Tregs decreased, but there was no significant statistical difference, while the change of Th2 cells was not obvious. Compared with model group and negative control group, the proportion of Th1 cells and Th17 cells in splenocytes of mice in HIF-1α-siRNA group decreased, and the difference was statistically significant (P<0.05), Although Th2 cells and Tregs showed an upward trend, the difference was not statistically significant. In model group, synovial hyperplasia and a large number of lymphocytes infiltrated in the ankle joint, after HIF-1α-siRNA treated CIA mice, synovial hyperplasia and the lymphocyte infiltration of mice ankle joint was significantly reduced. Conclusion: The proportion of Th1 cells and Th17 cells in the spleen of mice were reduced, the proportion of Th2 cells and Tregs in the spleen of mice were increased, synovial hyperplasia and lymphocytes infiltration were relieved. HIF-1α-siRNA play a role in the treatment of CIA mice by regulating T cell subsets in splenocytes.

Cite this article

MENG Jin , KANG Min , LU Yanrong , GUO Yu , PANG Chunyan . Regulation of hypoxia inducible factor-1 α on T cell subsets in splenocytes with collagen induced arthritis mice[J]. Journal of Baotou Medical College, 2023 , 39(5) : 17 -21 . DOI: 10.16833/j.cnki.jbmc.2023.05.004

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