Objective: To study the effect of total flavonoids of Rhizoma Drynariae (TFRD)on learning and memory impairment of sodium nitrite model mice. Methods: KM mice were randomly divided into the blank group, sodium nitrite model group 100 mg/(kg·d), Kangnaoshuai capsule group 585 mg/(kg·d), TFRD group 97.5 mg/(kg·d), and the TFRD+ER blocker group 97.5 mg/(kg·d)+0.072 mg/(kg·d). The learning and memory ability of mice were observed. Morphology of hippocampus was detected with HE staining. The contents of amyloid precursor protein (APP), amyloid precursor protein β-secretase (BACE1), over-phosphorylated tau protein (P-Tau396), cyclin-dependent protein kinase 5(CDK5), estrogen receptor β(ERβ) and phosphorylated p38(p-p38) in hippocampus were tested with Western blot. Results: The learning ability of mice in the model group decreased significantly. Granular cells in hippocampus were obviously necrotic. The expression levels of APP, BACE1, P-Tau396, CDK5 and P-P38/P38 in hippocampus increased significantly (P<0.01), while the expression level of ERβ decreased significantly (P<0.01). TFRD could improve the behavioral performance of mice in different models, decrease the expression levels of APP、BACE1、P-Tau396、CDK5 and P-P38/P38 (P<0.01), and increase the expression levels of ERβ. ER blocker could reverse the effect of TRFD on the above proteins. Conclusion: TFRD could improve learning and memory ability of mice in sodium nitrite model, which may due to the effect of TFRD on regulating proteins related to APP metabolic pathway.
WANG Yumo
,
LI Dalong
,
CUI Yue
,
ZHANG Yanhang
,
XU Hongdan
,
YANG Bo
,
SUN Huifeng
,
ZHANG Ning
. Improvement effects of total flavonoids of Rhizoma Drynariae on learning and memory in sodium nitrite model mice[J]. Journal of Baotou Medical College, 2023
, 39(5)
: 1
-5
.
DOI: 10.16833/j.cnki.jbmc.2023.05.001
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