Objective: To investigate the effect of hypoxia inducible factor-1α(HIF-1 α)on collagen induced arthritis (CIA) mice with interstitial lung disease (ILD) and T lymphocyte subsets in thymocytes by small interfering RNA (siRNA) vector of HIF-1α. Methods: CIA mice were established and divided into control group, CIA model group, negative control group and HIF-1α-siRNA group. The mice in control group were not treated, and the mice in negative control group were injected with empty adenovirus and those of HIF-1α-siRNA group were injected with HIF-1α-siRNA adenovirus, once a week for 4 weeks. After the experiment, all thymus were collected and thymocytes were cultured, and the changes of Th1 cells and Th2 cells, Th17 cells and Tregs in thymus were detected by flow cytometry, the pathological changes of mice lung tissue were observed by HE staining. Results: the proportion of Th1 cells and Th17 cells in thymocytes of CIA model group and negative control group increased and the proportion of Th2 cells and Tregs decreased, which was statistically significant compared with control group (P＜0.05). The proportion of Th1 and Th17 cells in thymocytes of mice in HIF-1α-siRNA group decreased, while the proportion of Th2 cells and Treg increased. Compared with CIA model group and negative control group, the difference was statistically significant (P＜0.05); There were a lot of lymphocyte infiltration, alveolar wall destruction and lung interstitial changes in the lungs of mice in CIA model group. After HIF-1α-siRNA treated CIA mice, the lymphocyte infiltration in the lungs of mice, the destruction of alveolar wall and the changes of lung stroma were alleviated. Conclusion: HIF-1α-siRNA can reduce the ratio of Th1 cells to Th17 cells and increase the ratio of Th2 cells and Tregs in thymocytes, reduce the lymphocyte infiltration in the lung tissue of CIA mice, and slow down the destruction of alveolar wall and the change of lung stroma, suggesting that HIF-1α-siRNA can treat CIA mice with interstitial lung disease by regulating T cell subsets in thymocytes.
KANG Min
,
MENG Jin
,
LU Yanrong
,
GUO Yu
,
PANG Chunyan
. Therapeutic effect of hypoxia inducible factor-1α on collagen induced arthritis associated-interstitial lung disease[J]. Journal of Baotou Medical College, 2023
, 39(3)
: 6
-9
.
DOI: 10.16833/j.cnki.jbmc.2023.03.002
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