Objective: To investigate the effect of long-term fluorine exposure on the expression of cysteinyl aspartate specific proteinase (caspase-1) and tumor necrosis factor-α (TNF-α) in rat myocardium. Methods: Wistar rats were randomly divided into the control group, the low-dose fluoride (50 mg/L) group, the middle-dose fluoride (100 mg/L) group and the high-dose fluoride (200 mg/L) group. The rats in the control group were fed with deionized water, and the rats in the fluoride groups were given deionized water containing different doses of sodium fluoride (NaF). Rats in each group were fed freely for 7 months to make an animal model of long-term fluoride exposure. Immunohistochemical staining was used to detect the expression level of myocardial Caspase-1 and TNF-α in long-term fluorine-exposed rats. Results: Compared with the rats in the control group, rats in the fluoride groups showed different degrees of dental fluorosis (DF), and the degree of fluorosis was dose - dependent with the dose of fluoride. The results of immunohistochemical staining showed that the expression levels of Caspase-1 and TNF-α in cardiomyocytes of rats stained with fluoride were increased (P＜0.05) and dose-dependent compared with rats in the control group. Conclusion: Long-term fluorine exposure may induce massive inflammatory factors in rat cardiomyocytes and cause cardiomyocyte damage by regulating caspase-1 level.
ZHANG Qiuyi
,
SHI Ruili
,
QI Ruifang
,
LV Jun
,
SHI Jinghua
,
WANG Lijun
,
MA Baohui
. Effects of long-term fluorine exposure on the expression of Caspase-1 and TNF-α of rat myocardium[J]. Journal of Baotou Medical College, 2023
, 39(2)
: 7
-11
.
DOI: 10.16833/j.cnki.jbmc.2023.02.002
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