Objective: To evaluate the effect of short-chain fatty acids (SCFAs) produced by intestinal flora on lipid metabolism in HepG2 cell lipid accumulation model. Methods: The lipid accumulation model of HepG2 cells was observed by oil red O staining. Different concentrations of SCFAs (acetic acid, propionic acid and n-butyric acid) were used to intervene the lipid accumulation model of HepG2 cells. The survival rate of HepG2 cells was detected by MTT method. TC and TG contents in HepG2 cell lipid accumulation model were detected by TC and TG kits. Western blot was used to detect the expression of AMPK, p-AMPK, ACC and p-ACC in HepG2 cells. Results: The activity of HepG2 cells gradually decreased with the increase of three concentrations of SCFAs ( P＜0.05). After intervention with SCFAs in the lipid accumulation model of HepG2 cells, the contents of TC and TG were decreased (P＜0.05), and the phosphorylation of AMPK and ACC in the lipid accumulation model group was inhibited. Conclusion: SCFAs affects the HepG2 cell activity, lipid metabolism and protein expression in HepG2 cell lipid accumulation model, and the intervention results of SCFAs under different concentrations and types are different.
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