Objective: To evaluate the content and antioxidant activity of total flavonoids from hawthorn leaves collected in Jinnan area of Shanxi. Methods: The content of total flavonoids from hawthorn leaves was determined by colorimetric analysis. The in vitro antioxidant activity of total flavonoids from hawthorn leaves was evaluated by determining their scavenging capacity against 1,1-diphenyl-2-picrylhydra-zyl (DPPH), 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and hydroxyl free radical. Results: The content of total flavonoids in hawthorn leaves was determined to be (79.06 ± 2.05) mg/g. The half maximal inhibitory concentration (IC50) values of total flavonoids from hawthorn leaves on DPPH and ABTS radical were (91.63 ± 1.75) and (64.34± 4.53) μg/mL respectively, with a relative antioxidant activity (RAA) of 40.71 % and 76.64 % apart. Compared with ascorbic acid, flavonoid from hawthorn leaves has a lower scavenging rate of hydroxyl free radical. The above three values of flavonoid from hawthorn leaves were all lower than that of ascorbic acid under the same mass concentration. Conclusion: Hawthorn leaves collected in Jinnan area of Shanxi have good content of total flavonoids and high antioxidant activity, further study should be performed to confirm its specific mechanism.
XUE Yutong
,
ZHENG Jinxiu
,
SHAO Ying
,
YANG Lijun
. Determination of the total flavonoid content in Hawthorn leaves and its antioxidant activity[J]. Journal of Baotou Medical College, 2023
, 39(1)
: 18
-22
.
DOI: 10.16833/j.cnki.jbmc.2023.01.005
[1] Liu J, Wang XC, Yong HM, et al. Recent advances in flavonoid-grafted polysaccharides: synthesis, structural characterization, bioactivities and potential applications[J]. Int J Biol Macromol, 2018, 116: 1011-1025.
[2] Abu H, Khalil R, Nazish J, et al. The role of polyphenol (flavonoids) compounds in the treatment of cancer cells[J]. Nutr Cancer, 2020, 72(3): 386-397.
[3] 陈皓, 王隆, 余静, 等. 山楂叶多糖和黄酮调节幼鼠的糖脂代谢和免疫功能研究[J]. 食品安全质量检测学报, 2021, 12(21): 8397-8403.
[4] 国家药典委员会. 中华人民共和国药典[S]. 北京: 中国医药科技出版社, 2020: 33.
[5] 王艳, 赵露, 徐惠波. 山楂叶总黄酮药理研究与临床应用进展[J]. 人参研究, 2015, 27(2): 54-59.
[6] 弓威, 顾丰颖, 贺凡, 等. 山楂叶有效成分提取工艺优化及抗氧化活性研究[J]. 核农学报, 2015, 29(8): 1547-1558.
[7] 杨志坚, 温杭凯, 陈选阳, 等. 藤茶总黄酮和二氢杨梅素含量研究[J]. 福建农业科技, 2017, (10): 6-9.
[8] 都启晶, 王艺颖, 丁君, 等. 大豆发芽过程中维生素C、异黄酮含量变化及其抗氧化性的研究[J]. 食品安全质量检测学报, 2015, 6(9): 3608-3613.
[9] 李艳荣, 周维维, 王子怡, 等. 山楂叶提取物抗氧化活性及谱效关系研究[J]. 中国药学杂志, 2020, 55(20): 1673-1679.
[10] 毛涵, 牛蕾, 薛菲, 等. 鸢尾叶异黄酮超声波辅助提取工艺优化及抗氧化活性研究[J]. 食品工业, 2017, 38(7): 148-152.
[11] 磨正遵, 商飞飞, 潘中田, 等. 响应面法优化超声波辅助提取广西大果山楂叶黄酮工艺[J]. 南方农业学报, 2018, 49(5): 986-992.
[12] 许瑞波, 杨雯雯, 万蓓蓓. 连云港山楂叶黄酮的超声提取及体外活性研究[J]. 井冈山大学学报(自然科学版), 2016, 37(6): 24-29.
[13] 宋小锋, 原增艳, 申笑菡, 等. 山楂叶总黄酮富集工艺的优化及其清除自由基能力的检测[J]. 中国生物制品学杂志, 2020, 33(2): 184-188.
[14] 向小林. 不同时期山楂叶黄酮含量及抗氧化活性变化[D]. 秦皇岛: 河北科技师范学院, 2013.
[15] 宋璇,王汝华,于建丽,等.山楂叶黄酮分离纯化及抗氧化活性[J].食品研究与开发,2022,43(4):57-63.
