Objective: To evaluate the content and antioxidant activity of total flavonoids from hawthorn leaves collected in Jinnan area of Shanxi. Methods: The content of total flavonoids from hawthorn leaves was determined by colorimetric analysis. The in vitro antioxidant activity of total flavonoids from hawthorn leaves was evaluated by determining their scavenging capacity against 1,1-diphenyl-2-picrylhydra-zyl (DPPH), 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and hydroxyl free radical. Results: The content of total flavonoids in hawthorn leaves was determined to be (79.06 ± 2.05) mg/g. The half maximal inhibitory concentration (IC50) values of total flavonoids from hawthorn leaves on DPPH and ABTS radical were (91.63 ± 1.75) and (64.34± 4.53) μg/mL respectively, with a relative antioxidant activity (RAA) of 40.71 % and 76.64 % apart. Compared with ascorbic acid, flavonoid from hawthorn leaves has a lower scavenging rate of hydroxyl free radical. The above three values of flavonoid from hawthorn leaves were all lower than that of ascorbic acid under the same mass concentration. Conclusion: Hawthorn leaves collected in Jinnan area of Shanxi have good content of total flavonoids and high antioxidant activity, further study should be performed to confirm its specific mechanism.
XUE Yutong
,
ZHENG Jinxiu
,
SHAO Ying
,
YANG Lijun
. Determination of the total flavonoid content in Hawthorn leaves and its antioxidant activity[J]. Journal of Baotou Medical College, 2023
, 39(1)
: 18
-22
.
DOI: 10.16833/j.cnki.jbmc.2023.01.005
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