Objective: To investigate the effects of Icariin (ICA) on the proliferation and osteogenic differentiation of cementoblasts (OCCM-30) in vitro. Methods: OCCM-30 cells were cultured in vitro and added with different concentrations (10, 20, 40 μmol/L) of ICA and observed the cell morphology under inverted microscope. OCCM-30 cells was cultured in vitro and treated with blank control group and 10 μmol/L ICA group. The proliferation rate of OCCM-30 cells was detected by MTT method for 7 days. Real-time PCR was used to study the expression of osteogenic-related factors OCN and Runx-2 after 10 μmol/LICA was applied to OCCM-30 cells (1 d, 4 d, 7 d). Result: The results showed that there was no significant change in the morphology of OCCM-30 cells after adding ICA. MTT results showed that 10 μmol/L ICA significantly promoted the proliferation of OCCM-30 cells (P＜0.05). Real-time PCR results showed that the expression of osteogenic related factors OCN and Runx-2 in the experimental group was significantly higher than that in the control group (P＜0.05). Conclusion: Appropriate concentration of ICA can effectively promote the proliferation and osteogenic differentiation of OCCM-30 cells in vitro.
CHANG Qiufang
,
LIU Yan
,
YAO Yanan
,
LIANG Hongyan
,
XU Feng
,
WANG Jingyu
. Effects of Icariin on proliferation and osteogenic differentiation of cementoblasts in vitro[J]. Journal of Baotou Medical College, 2023
, 39(1)
: 13
-17
.
DOI: 10.16833/j.cnki.jbmc.2023.01.004
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