Objective: To observe the apoptosis of gastric cancer BGC-823 cells induced by cathepsin B (CTSB) combined with TRAIL. Methods: BGC-823 cells were routinely subcultured. The proliferation of BGC-823 cells was detected with MTT Cell Proliferation Assay Kit. The apoptosis rate was detected by flow cytometry (FCM) and the expression level of cathepsin B protein was detected with Western blot. Results: TRAIL could inhibit the proliferation of BGC-823 cells and promote apoptosis(P＜0.05). The higher concentration of cathepsin B inhibitor could lead to the lower apoptosis rate(P＜0.05). Western blot results showed that the higher concentration of cathepsin B inhibitor would cause the lower expression of cathepsin B(P＜0.05). Conclusion: Cathepsin B combining with TRAIL could synergistically induce apoptosis of gastric cancer BGC-823 cells.
ZHA Daode
,
TU Longxia
,
WU Wenyong
. Role of cathepsin B combining with TRAIL on inducing apoptosis in gastric cancer BGC-823 cells[J]. Journal of Baotou Medical College, 2023
, 39(1)
: 4
-7
.
DOI: 10.16833/j.cnki.jbmc.2023.01.002
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