Objective: To study the effects of diammonium glycyrrhizinate (DGLL) on acute lung injury (ALI) and pulmonary edema in rats. Methods: A total of 123 male SD rats, weighing 170~200 g, (6±1) weeks old, were intragastrically administrated with DGLL (30, 60, 120 mg/kg). After 1 h, the ALI model was established by intraperitoneal injection of LPS (10 mg/kg ). After 6 h, the lung injury was evaluated by HE staining. Lung wet to dry weight ratio, protein content in bronchoalveolar lavage fluid (BALF) and Evans blue (EB) in lung tissue were used to evaluate pulmonary edema. The expression levels of tumor necrosis factor (TNF-α) and interleukin (IL-1β) in lung tissue were determined by ELISA. The expression level of myeloperoxidase (MPO) was detected by immunohistochemical staining. The expression levels of intercellular adhesion molecule (ICAM-1), vascular endothelial cadherin (VE-cadherin), adhesion protein (ZO-1), and connective adhesion molecule (JAM-1) related to lung inflammation and microvascular permeability were determined by Western blotting. Results: The immunoreactivity of MPO was decreased, and the expression levels of TNF-α, IL-1β and ICAM-1 in lung tissue of rats were decreased, indicating that DGLL alleviated LPS-induced ALI. In addition, DGLL could inhibit LPS-induced pulmonary edema, reduce BALF protein concentration and EB extravasation. DGLL also could reduce the expression level of VE-cadherin in endothelial cells and inhibited the expression of connexins in lung tissue induced by LPS, including ZO-1 and JAM-1. Conclusion: DGLL has a protective effect on LPS-induced ALI in rats, and also inhibits the infiltration of inflammatory cells and the destruction of microvascular barrier.
LIU Meimei
,
HUANG Yanping
,
JI Dan
,
WANG Qi
. The improvement of diammonium glycyrrhizinate lipid ligand on acute lung injury in rats[J]. Journal of Baotou Medical College, 2022
, 38(11)
: 91
-96
.
DOI: 10.16833/j.cnki.jbmc.2022.11.018
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