Objective: To explore the expression changes of glutamate decarboxylase 67 (GAD67) and m-opioid receptor (MOR) in thalamic reticular nucleus (TRN) after rat intracerebral hemorrhage model (ICH). Methods: The right ventrolateral thalamic nucleus (VPL) was injected with type IV collagenase under the stereotaxic apparatus to establish the rat thalamus hemorrhage model. The same volume of normal saline was injected at the same position as the control group. The morphology of neurons around VPL was observed by Nissl staining, and the changes of GAD67 and MOR contents in TRN were observed by immunofluorescence staining. Results: No obvious abnormality of motor function was found in the thalamic hemorrhage model of VPL injection; Nissl body necrosis, atrophy and vacuolar degeneration of neurons in VPL of model group; In the control group, the number of MOR in TRN was 77.77±9.45, decreased to 30.00±4.90 at 7 days after ICH (P＜0.05), 23.30±2.05 at 14 days after ICH (P＜0.05), and 10.50±2.87 at 28 days after ICH (P＜0.05). The number of GAD67 positive cells in the control group was 78.67±7.77, which was 27.00±5.35 at 7 days after ICH (P＜0.05), 41.67±5.44 at 14 days after ICH (P＜0.05), and 26.00±6.13 at 28 days after ICH (P＜0.05). Conclusion: VPL injection of type IV collagenase in rats can successfully prepare the thalamus hemorrhage model. The motor function of rats in the model group has no significant change. The GAD67 protein in the TRN of rats in the model group is reduced, and the number of MOR is reduced.
FENG Chengming
,
KOU Zhenzhen
,
ZHOU Hongying
,
LI Yunqing
,
CAI Zhiping
. Changes of GAD67 and MOR receptor contents in thalamus reticular nucleus after thalamus hemorrhage in rats[J]. Journal of Baotou Medical College, 2022
, 38(11)
: 34
-38
.
DOI: 10.16833/j.cnki.jbmc.2022.11.007
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