Objective: To establish the quality standard of the Mongolian medicine Sanhuang ointment. Methods: Thin layer chromatography (TLC) was used to qualitatively identify Coptidis Rhizoma and Phellodendri Chinese Cortex in the Mongolian medicine preparation Sanhuang ointment; high performance liquid chromatography (HPLC) was used to determine the content of Berberine hydrochloride in the preparation. Results: In the TLC method, the samples showed spots of the same color on the corresponding positions of the chromatograms of the control herbs or controls, and there was no interference in the negativity. Berberine hydrochloride showed good linearity in the range of 45.72 -274.34 μg/mL, the regression equation was y=0.1643x+6.7144, R2=0.9992, the average recovery was 94.16 %. Conclusion: The TLC qualitative identification of Coptidis Rhizoma and Phellodendri Chinese Cortex is highly specific; the method for the determination of berberine hydrochloride is highly specific, accurate, stable, sensitive, simple and fast, and other components in the formula do not interfere with its determination. The standard established in this study can effectively control the quality of Mongolian medicine Sanhuang ointment.
WEI Jinhui
,
TAN Yimeng
,
WU Lijun
,
YANG Dan
,
BO Yukun
,
AN Ming
. Study on the quality standard of the Mongolian medicine Sanhuang ointment[J]. Journal of Baotou Medical College, 2022
, 38(10)
: 74
-78
.
DOI: 10.16833/j.cnki.jbmc.2022.10.015
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