Objective: To observe the expression changes of FGF2 in mouse hippocampal neurons with hypoxia and hypoxia preconditioning, and to explore whether FGF2 participates in the neuroprotection of hypoxia tolerance. Methods: Bioinformatics methods were used search and identify FGF2 gene family members. At the cellular level, establish hypoxia and hypoxic preconditioning models, and detect FGF2 expression in cells by Western-blot and qRT-PCR. Results: The FGFs family is divided into 7 sub-families, among which FGF2 contains an FGF receptor binding domain composed of 17 amino acids, which can bind to specific FGF receptors for signal transduction. At the cell level, compared with control group, the expression of FGF2 mRNA and protein in H group and HPC group was significantly decreased (P＜0.05), while compared with H group, the expression of FGF2 mRNA and protein of HPC group was increased (P＜0.05). Conclusion: FGF participates in the neuroprotective effect of hypoxic preconditioning.
CAO Xiaoyu
,
YANG Haixia
,
JIN Qingling
,
XIE Wei
,
SHAO Guo
,
BAO Mulan
,
BADE Rengui
. Bioinformatics analysis of FGF2 and its role in hypoxic neuroprotection[J]. Journal of Baotou Medical College, 2022
, 38(10)
: 1
-5
.
DOI: 10.16833/j.cnki.jbmc.2022.10.001
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