Objective: To investigate the effects of Astragalus mongholicus polysaccharides on apoptosis of prefrontal neurons and BDNF / TrkB / CREB signaling pathway in ADHD model rats (SHR). Method: Twenty 4-week old SHR rats were divided into model group, Mongolian astragalus polysaccharide group (polysaccharide group), Jingling oral liquid group (Jingling group), methylphenidate group, and five Wistar rats were set as control group. After four weeks of gavage, water maze test and open field test were performed, and the animals were killed.Blood was taken from abdominal aorta after anesthesia, and determination of Inositol Triphosphate (IP3), FosB and c-Fos in serum by ELISA.After execution, the prefrontal lobe was taken for Nissl staining and Real Time PCR to detect BDNF, TrkB, CREB mRNA in the prefrontal lobe. Results: In the water maze test, there was no significant difference in the behavior of rats in each group (P＞0.05). The standing times of polysaccharide group, methylphenidate group and Jingling group were reduced compared with the model group (P＜0.05), and the hair dressing times of polysaccharide group and Jingling group were reduced compared with the model group (P＜0.05). Compared with the other four groups, the structure of neurons in the model group was incomplete, and the nuclear membrane was obviously fragmented and severely damaged.IP3 in polysaccharide group and methylphenidate group was lower than that in model group (P＜0.05).Compared with model group, c-Fos in polysaccharide group, methylphenidate group and Jingling group decreased (P＜0.05).There was no significant difference in FosB among groups (P＞0.05).The expression level of BDNF mRNA in polysaccharide group and Jingling group was higher than that in model group (P＜0.05), while that of CREB mRNA in methylphenidate group and Jingling group was higher than that in model group (P＜0.05). Conclusion: The behavioral changes of SHR rats in the open field experiment may be related to the regulation of the expression of BDNF mRNA in the prefrontal lobe of SHR rats by astragalus mongholicus polysaccharides, the reduction of IP3, c-Fos levels and the reduction of the apoptosis of prefrontal neurons.
LI Xin
,
WEI Litao
,
ZHAO Binghong
,
GAO Lei
,
GUAN mingjie
. Effect of Astragalus mongholicus polysaccharides on prefrontal neurons in ADHD rat[J]. Journal of Baotou Medical College, 2022
, 38(12)
: 11
-16
.
DOI: 10.16833/j.cnki.jbmc.2022.12.003
[1] Oh H, Lewis DA, Sibille E. The role of BDNF in age-dependent changes of excitatory and inhibitory synaptic markers in the human prefrontal cortex[J]. Neuropsychopharmacology, 2016,41(13):3080-3091.
[2] Juhasz G, Dunham JS, Mckie S, et al. The CREB1-BDNF-NTRK2 pathway in depression: multiple gene-cognition-environment interactions[J]. Biol Psychiatry, 2011,69(8):762-771.
[3] Nagib MM, Tadros MG, Rahmo RM, et al. Ameliorative effects of α-tocopherol and/or coenzyme Q10 on phenytoin-induced cognitive impairment in rats: role of VEGF and BDNF-TrkB-CREB pathway[J]. Neurotox Res, 2019,35(2):451-462.
[4] Wang HT, Xu JP, Lazarovici P, et al. cAMP response element-binding protein (CREB):a possible signaling molecule link in the pathophysiology of schizophrenia[J]. Front Mol Neurosci, 2018,11:255.
[5] 周劭华,高磊,赵秉宏,等.蒙古黄芪有效成分对注意缺陷多动障碍模型大鼠行为学及神经递质的影响[J].中华中医药学刊,2020,38(2):84-87.
[6] Yasumura A, Omori M, Fukuda A, et al. Age-related differences in frontal lobe function in children with ADHD[J]. Brain Dev, 2019,41(7):577-586.
[7] Yin JB, Liang SH, Li F, et al. dmPFC-vlPAG projection neurons contribute to pain threshold maintenance and antianxiety behaviors[J]. J Clin Invest, 2020,130(12):6555-6570.
[8] 周家林,慕泽泾,钟国跃,等.黄芪属植物化学成分及生物活性研究进展[J].中成药,2021,43(7):1845-1851.
[9] 向艳霞,肖凌云,张菊,等.黄芪多糖治疗神经系统疾病的作用及其机制研究进展[J].中国医院药学杂志,2016,36(8):687-691.
[10] 宿宏佳,王冬梅,张腾,等.黄芪多糖对抑郁大鼠海马Nrf2-ARE通路的影响[J].中国药理学通报,2021,37(6):839-843.
[11] 兰红艳,吴丹,汪秀莲,等.基于近红外光谱的注意缺陷多动障碍患儿前额叶皮质功能研究[J]. 中国儿童保健杂志,2021,29(2):134-137.
[12] Xiao P, Liu XW, Zhao NN, et al. Correlations of neuronal apoptosis with expressions of c-Fos and c-Jun in rats with post-ischemic reconditioning damage[J]. Eur Rev Med Pharmacol Sci, 2018,22(9):2832-2838.
[13] Chase TD, Carrey N, Brown RE, et al. Methylphenidate differentially regulates c-fos and fosB expression in the developing rat striatum[J]. Brain Res Dev Brain Res, 2005,157(2):181-191.
[14] 张迎春,朱学清,张雪寒.利他林对幼年大鼠额叶皮层小清蛋白阳性中间神经元c-Fos表达的影响[J].生理学报,2017,69(4):378-384.
[15] Colucci-D'Amato L, Speranza L, Volpicelli F. Neurotrophic factor BDNF, physiological functions and therapeutic potential in depression, neurodegeneration and brain cancer[J]. Int J Mol Sci, 2020,21(20):7777.
[16] Sonoyama T, Stadler LKJ, Zhu MY, et al. Human BDNF/TrkB variants impair hippocampal synaptogenesis and associate with neurobehavioural abnormalities[J]. Sci Rep, 2020,10(1):1-14.
[17] Yoshii A, Constantine-Paton M. Postsynaptic BDNF-TrkB signaling in synapse maturation, plasticity, and disease[J]. Dev Neurobiol, 2010,70(5):304-322.
[18] Du Q, Zhu XY, Si JR. Angelica polysaccharide ameliorates memory impairment in Alzheimer's disease rat through activating BDNF/TrkB/CREB pathway[J]. Exp Biol Med (Maywood), 2020,245(1):1-10.
