Objective: to establish the model of distal middle cerebral artery occlusion (Distal middle cerebral artery occlusion,dMCAO) in C57BL/6 mice by electrocoagulation and to improve the mouse model of acute ischemic stroke (Stroke). Methods: 54 male C57BL/6 mice aged 8-10 weeks were randomly divided into normal group (n=6), sham operation group (n=6) and dMCAO model group (n=42).The model was established by double electrocoagulation of one side of the cortical branch of the middle cerebral artery and ligation of the ipsilateral common carotid artery.The survival rate of 3 days and 7 days and the success rate of 7 days were evaluated; the proportion of cerebral infarction volume was evaluated by TTC staining on 7 days; the cerebral blood flow was evaluated by laser microcirculation blood flow imaging; and the motor coordination ability of mice was evaluated by rotating stick test on 3 days and 7 days. Results: the 3-day and 7-day survival rate of normal group and sham operation group was 100 %.The average survival rate of 3-day and 7-day model group was 83.35 %, 78.43 % and 66.7 %, respectively.The infarct volume of model mice was evaluated by TTC staining.The proportion of infarct volume in normal group and sham operation group was 0 %.The proportion of infarct volume in MCAO model group was (18.22 ±0.89) %, and the confidence interval of 95 % was (17.28,19.15) %.The cerebral blood flow of model mice was evaluated by laser microcirculation blood flow imager.there was no significant difference in the proportion of bilateral cerebral blood flow between the normal group and the sham operation group.In the dMCAO model group, the infarcted cerebral blood flow decreased by (53.85 ±1.22) % and 95 % confidence interval (52.57,55.13) % compared with the contralateral cerebral blood flow in the dMCAO model group (n=6).The motor ability of the model mice was evaluated by the stick time in the stick test, there was no significant difference between the normal group and the sham operation group, but there was significant difference between the dMCAO model group (n=6) and the sham operation group. Conclusion: the reliable dMCAO model of C57BL/6 mice can be established by double electrocoagulation of unilateral middle cerebral artery and ligation of ipsilateral common carotid artery.
KANG Min
,
XUE Hui
,
ZHANG Xin
. Establishment and evaluation of DMCAO model in C57BL/6 mice[J]. Journal of Baotou Medical College, 2022
, 38(12)
: 5
-10
.
DOI: 10.16833/j.cnki.jbmc.2022.12.002
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