Objective: To analyze the application of Amikacin injection in the outpatient and emergency department in the hospital and its rationality.Methods: 3 499 prescriptions of outpatient and emergency from January 2016 to December 2016 in the hospital were selected. Statistical analysis was made on the application of Amikacin injection from different age groups, the number of single use and people counting, the number of prescriptions and the clinical diagnosis types.Results: A total of 3 499 electronic prescriptions were investigated, with a total of 2061 patients. Among them, 0-8 years old children had 3078 prescriptions, accounting for 88.0 %. 3 499 prescriptions in outpatient and emergency departments were made by 12 doctors, in which a doctor made 2134 prescriptions, accounting for 61.0 %. Respiratory diseases were the main diagnosis, accounting for 90.0 %, in which upper respiratory tract diseases accounted for 42.1% and lower respiratory tract diseases accounted for 47.9 %.Conclusion: In 2016, the unreasonable phenomenon of Amikacin injection application for 0-12 years old children in outpatient and emergency department in the hospital exist, and thus the function of clinicians should be enhanced and the clinical rational use of drugs should be further promoted.