Objective: To investigate the application value of high-risk human papilloma virus (HPV) test combined with thin-cytologic test (TCT) detection in cervical cancer screening.Methods: 121 cases of patients under cervical cancer screening in the hospital were selected and given high-risk HPV test and TCT detection respectively. The results of colposcopic histopathological biopsy were taken as diagnostic standard, and the results of TCT detection alone, high-risk HPV test alone and high-risk HPV test combined with TCT detection were compared.Results: The accuracy of the result of high-risk HPV combined with TCT detection significantly increased compared with that of TCT detection alone or high-risk HPV test alone in the detection rate of cervical cancer (P＜0.05); The sensitivity, specificity, positive predictive value and negative predictive value of high-risk HPV combined with TCT detection were significantly better than those of HPV test alone or TCT detection alone (P＜0.05).Conclusion: High-risk HPV test combined with TCT detection in the diagnosis of cervical cancer has higher accuracy, sensitivity and specificity, which is worthy of clinical promotion.