目的: 观察沙棘总黄酮(TFH)对特应性皮炎(AD)模型小鼠炎症反应的干预作用。方法: 将BALB/c小鼠随机分为正常对照组、AD模型组和TFH干预组,每组10只。对AD模型组和TFH干预组小鼠左耳背部皮肤涂抹2 nmol/L MC903,对正常对照组小鼠涂抹等体积无水乙醇,每天1次,共14天。从第7天开始,对TFH干预组小鼠左耳背部皮肤涂抹1%TFH乳剂20 μg,每天1次,共8天。监测左耳厚度并评估皮损严重程度,取引流淋巴结称重,采用苏木素-伊红(HE)染色和甲苯胺蓝(TB)染色法观察皮损部位形态改变,统计相关形态学参数,检测血清中IgE、IL-6、MDC水平。结果: 与AD模型组相比,TFH干预组炎症评分显著降低(P＜0.01),左耳皮肤全层以及皮肤上皮层的厚度均呈现出明显的变薄(P＜0.01),皮肤真皮层炎症细胞浸润减轻、肥大细胞数量显著减少(P＜0.01),引流淋巴结重量显著减轻(P＜0.01),血清IgE、IL-6、MDC水平显著降低(P＜0.01)。结论: 外用TFH能够降低AD模型小鼠的炎症反应。
Objective: To observe the intervention effect of total flavonoids of Hippophae rhamnoides L. (TFH) on inflammatory response in atopic dermatitis (AD) model mice. Mechods: BALB/c mice were randomly divided into normal control group, AD model group and TFH intervention group, 10 mice in each group. The mice in the AD model group and TFH intervention group were treated with 2 nmol/L MC903 on the back skin of the left ear once a day for 14 days, and the mice in the normal control group were treated with the same volume of absolute ethanol once a day for 14 days. From the 7th day, 1% TFH emulsion 20 μg was applied to the left ear back skin of mice in the TFH intervention group for 8 days. The thickness of the left ear was monitored and the severity of the lesion was evaluated. The draining lymph nodes were weighed, and the morphological changes of the lesion were observed by hematoxylin-eosin (HE) staining and toluidine blue (TB) staining. The morphological parameters were counted, and the levels of IgE, IL-6 and MDC in serum were detected. Results: Compared with the AD model group, the inflammation score of the TFH intervention group was significantly reduced (P＜0.01), the thickness of the whole layer of the left ear skin and the skin epithelium showed a significant thinning (P＜0.01), the infiltration of inflammatory cells in the dermis of the skin was reduced, the number of mast cells was significantly reduced (P＜0.01), the weight of the draining lymph nodes was significantly reduced (P＜0.01), and the levels of serum IgE, IL-6 and MDC were significantly reduced (P＜0.01). Conclusion: External application of TFH can reduce the inflammatory response in AD model mice.
[1] Guttman-Yassky E, Renert-Yuval Y, Brunner PM. Atopic dermatitis[J]. Lancet, 2025, 405(10478): 583-596.
[2] Kircik LH. Management of Atopic Dermatitis[J]. Drugs Dermatol, 2019, 18, s111.
[3] Jing D, Li J, Tao J. et al. Associations of second-hand smoke exposure with hand eczema and atopic dermatitis among college students in China[J]. Sci Rep, 2020, 10: 17400.
[4] 雷邱添, 杨滨宾, 邓丹琪. 特应性皮炎与心血管疾病的相关性[J]. 中国皮肤性病学杂志, 2025, 39(6): 591-595.
[5] 国家药典委员会. 中华人民共和国药典[M]. 2005年版一部. 北京: 化学工业出版社, 2005, 127-128.
[6] Qi F, Sun JH, Yan JQ, et al. Anti-inflammatory effects of isorhamnetin on LPS-stimulated human gingival fibroblasts by activating Nrf2 signaling pathway[J]. Microb Pathog, 2018, 120: 37-41.
[7] Larmo P, Alin J, Salminen E, et al. Effects of sea buckthorn berries on infections and inflammation: a double-blind, randomized, placebo-controlled trial[J]. Eur J Clin Nutr, 2008, 62: 1123-1130.
[8] Л.Н.Юдкина, 孙约翰. 苏联药植医疗经验介绍应用沙棘油治疗几种皮肤病的经验[J]. 上海中医药杂志, 1955, (12): 43-44.
[9] 张培波, 李义. 沙棘总黄酮抑制膀胱癌细胞系T24增殖[J]. 基础医学与临床, 2023, 43(7): 1122-1126.
[10] 徐炫, 谌燕, 陈荫. 沙棘枝干总黄酮的提取和抗氧化活性研究[J]. 安徽农业科学, 2025, 53(11): 172-180.
[11] 贾丛, 杜亚蓉, 孙坤. 沙棘总黄酮抑制肺癌A549增殖和迁移作用及机理[J]. 天然产物研究与开发, 2020, 32(6): 937-945, 952.
[12] 练霓, 史丽晴, 刘力豪, 等. 皮肤屏障与病毒感染的研究进展[J]. 中国麻风皮肤病杂志, 2021, 37(1): 50-55.
[13] 王欣欣. 基于Th1/Th2平衡研究沙棘果油及其有效成分对特应性皮炎小鼠的干预作用[D].沈阳:辽宁中医药大学, 2020.
[14] Altaii Hiyam A, AlTae Firas MD. Investigation of Serum Total IgE and Eosinophil Levels in Different Allergic Diseases Together with the Study of Their Correlations with Various Possible Allergens in Mosul City[J]. Immunol Invest, 2020, 51(3): 21-26.
[15] 冯强, 陈红梅, 唐瑞莲, 等.盆炎1号方经皮经穴靶向透药对慢性盆腔炎患者IL-10、IL-6、MMP-9及免疫功能的影响[J]. 中国免疫学杂志, 2024, 40(8): 1735-1739.
[16] 何江, 邱丹, 曾红英.血清PCT、IL-6与急性心肌梗死伴肺部感染患者预后不良的相关性[J].中国老年学杂志, 2024, 44(24): 5892-5896.
[17] 朱梦远, 李晓照, 周巧玲, 等. IgA肾病伴扁桃体炎患者Th22细胞及CCL27/CCR10轴的表达[J]. 中华肾脏病杂志, 2020, (4): 271-277.
[18] Xiaomeng X, Louise SG, Mark JAK. Factors influencing quality of life in children with atopic dermatitis and their caregivers: a cross-sectional study[J]. Sci Rep, 2019, 9(1): 15990.
[19] 胡宇晴, 刘萍, 王雨馨, 等.我国皮肤科医生的特应性皮炎用药选择情况分析[J]. 中国皮肤性病学杂志, 2024, 38(7): 748-754.
