目的:探究高浓度人绒毛膜促性腺激素(HCG)对小鼠卵巢生长发育及卵泡凋亡的调控作用。方法:选取产后10 d的健康雌鼠(20只),采用随机数字表法均分为HCG注射组(10只)与对照组(10只),HCG注射组腹腔注射5 IU HCG 0.1 mL,对照组注射等量生理盐水,连续注射3 d。最后一次注射后间隔12 h收集卵巢组织,进行卵巢切片、卵泡分级计数、卵巢细胞凋亡相关基因Bax、Bcl2、caspase-3的转录水平及Bax和Bcl2的蛋白水平表达检测。结果:苏木精染色结果与卵泡分级计数结果表明,与对照组相比,HCG注射组原始卵泡数量显著减少(P＜0.05),而生长卵泡(初级及次级卵泡)计数显著增加(P＜0.05)。同时,HCG注射组卵巢组织中凋亡相关基因Bax、Bcl2、caspase-3的转录水平及Bax和Bcl2的翻译水平显著下调(P＜0.05)。结论:注射高浓度HCG后小鼠原始卵泡凋亡减少并可能促进原始卵泡激活为生长卵泡。
Objective: To investigate the regulatory effects of high-concentration human chorionic gonadotropin (HCG) on ovarian development and follicular apoptosis in mice. Methods: Twenty healthy female mice at 10 days postpartum were selected and randomly divided into the HCG injection group (n=10) and the control group (n=10) using a random number table method. Mice in the HCG injection group were intraperitoneally injected with 0.1 mL of 5 IU HCG, while those in the control group were injected with an equal volume of normal saline. The injections were administered continuously for 3 days. Ovarian tissues were collected at 12 hours after the last injection. Subsequent analyses included preparation of ovarian sections, grading and counting of follicles, detection of the transcriptional levels of ovarian cell apoptosis-related genes (Bax, Bcl2, and caspase-3), as well as determination of the protein expression levels of Bax and Bcl2. Results: The results of HE staining and counting showed that, compared with the control group, the number of primordial follicles in the HCG injection group was significantly reduced (P＜0.05), while the count of growing follicles (primary and secondary follicles) was significantly increased (P＜0.05). Meanwhile, in the ovarian tissues of the HCG injection group, the transcriptional levels of apoptosis-related genes Bax, Bcl2, and caspase-3, as well as the translational levels of Bax and Bcl2, were significantly down-regulated (P＜0.05). Conclusion: After injection of high concentration of HCG, the apoptosis of primordial follicles in mice decreased and may promote the activation of primordial follicles into growing follicles.
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