目的: 探讨受体相互作用蛋白激酶3(RIPK3)在胃腺癌及癌前病变组织中的表达及其临床意义。方法: 收集包头医学院第二附属医院2022年12月至2024年6月期间采集的170例胃腺癌及癌前病变组织病理样本(包括40例慢性浅表性胃炎、40例慢性萎缩性胃炎伴肠化生、40例异型增生和50例胃腺癌),使用免疫组化法检测RIPK3的表达;收集30例新鲜组织样本,利用Western blot检测RIPK3的表达情况;通过Kaplan-Meier plotter分析RIPK3表达与胃腺癌患者生存期的关系,并使用TISCH数据库探讨RIPK3与胃癌肿瘤微环境(TME)的关联。结果: (1)免疫组化显示,RIPK3在胃腺癌中的阳性表达率显著低于其他组(P＜0.05),且与肿瘤浸润深度、TNM分期、脉管侵犯和淋巴结转移相关(P＜0.05)。(2)Western blot结果显示,RIPK3在慢性浅表性胃炎、慢性萎缩性胃炎伴肠化生和胃腺癌中的表达逐渐降低(P＜0.01)。(3)Kaplan-Meier分析表明,RIPK3高表达的胃腺癌患者总生存期(OS)和无进展生存期(FP)较长。(4)TME分析发现,RIPK3表达与细胞间质及黏液分泌密切相关。结论: RIPK3低表达与胃腺癌的发生、浸润、转移及预后不良相关。
Objective: To investigated the expression of receptor-interacting protein kinase 3 (RIPK3) in gastric adenocarcinoma and precancerous tissues. Methods: The study focuses on 170 pathological samples of gastric adenocarcinoma and precancerous lesions collected from the Second Affiliated Hospital of Baotou Medical College from December 2022 to June 2024, pathological samples including 40 cases of chronic superficial gastritis, 40 cases of chronic atrophic gastritis with intestinal metaplasia, 40 cases of heterotrophic hyperplasia and 50 cases of gastric adenocarcinoma. To use immunohistochemistry to detect the expression of RIPK3. Meanwhile, 30 fresh tissue samples are collected to detect the expression of RIPK3 using Western blot. The relationship between RIPK3 expression and survival of gastric adenocarcinoma patients is analysed by Kaplan-Meier plotter, and the association between RIPK3 and the tumour microenvironment (TME) of gastric cancer is explored using TISCH database. Results: (1) Immunohistochemistry shows that the positive expression rate of RIPK3 in gastric adenocarcinoma is significantly lower than that in other groups (P＜0.05) and correlated with the depth of tumour infiltration, TNM stage, vascular invasion and lymph node metastasis (P＜0.05). (2) Western blot results shows that the expression of RIPK3 in chronic superficial gastritis, chronic atrophic gastritis with intestinal metaplasia and gastric adenocarcinoma gradually decreased (P＜0.01). (3) Kaplan-Meier analysis shows that gastric adenocarcinoma patients with high RIPK3 expression has longer overall survival (OS) and progression-free survival (FP). (4) TME analysis reveals that RIPK3 expression is closely associated with intercellular stroma and mucus secretion. Conclusion: Low expression of RIPK3 is associated with gastric adenocarcinoma occurrence, infiltration, metastasis and poor prognosis.
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