目的：探讨环状 RNA中的CircRNA-IFT80在急性脑梗死患者外周血中的表达水平及临床价值。方法：选择2021年6月至2021年12月连续收入院的急性脑梗死患者39例为研究组,选取同一医院相同时期的就诊或体检人群44例为对照组。采用实时荧光定量PCR法检测CircRNA-IFT80水平,分析基本资料和CircRNA-IFT80表达水平与急性脑梗死的相关性。运用ROC曲线分析CircRNA-IFT80在急性脑梗死中的价值。结果：研究组循环血中Circ RNA-IFT80的表达低于健康对照组(P＜0.001)。急性脑梗死的发生与年龄、性别、收缩压、舒张压、总胆固醇、高密度脂蛋白、高血压史、吸烟史、饮酒史、CircRNA-IFT80的表达有显著性相关。CircRNA-IFT80的表达量为急性脑梗死发病的保护性因素。结论：Circ RNA-IFT80在脑梗死中表达差异,Circ RNA-IFT80可在急性脑梗死的发生发展中发挥潜在作用。
Objective: To investigate the expression level and clinical value of CircRNA-IFT80 in peripheral blood of patients with acute cerebral infarction. Methods: From June 2021 to December 2021, 39 patients with acute cerebral infarction who were admitted to the hospital continuously were selected as the study group, and 44 patients who visited or underwent physical examination in the same hospital during the same period were selected as the control group. The level of CircRNA-IFT80 was detected by real-time fluorescence quantitative PCR, and the correlation between basic data and CircRNA-IFT80 expression level and acute cerebral infarction was analyzed. ROC curve was used to analyze the value of CircRNA-IFT80 in acute cerebral infarction. Results: The expression of Circ RNA-IFT80 in circulating blood of the study group was lower than that of the healthy control group (P＜0.001). The incidence of acute cerebral infarction was significantly correlated with age, sex, systolic blood pressure, diastolic blood pressure, total cholesterol, high density lipoprotein, hypertension history, smoking history, drinking history and CircRNA-IFT80 expression. The expression of CircRNA-IFT80 was a protective factor for acute cerebral infarction. Conclusion: The expression of Circ RNA-IFT80 is different in cerebral infarction, and Circ RNA-IFT80 may play a potential role in the occurrence and development of acute cerebral infarction.
[1] Jiang W, Long X, Li Z, et al. The role of circular RNAs in ischemic stroke[J]. Neurochem Res, 2023,48(9):2607-2620.
[2] Chen Y, Wang B, Liu W, et al. Diagnostic Value of Serum hsa_circ_0141720 in Patients with Acute Ischemic Stroke[J]. Clin Lab, 2020,66(8):191266.
[3] 陈静燕,符丽,董瑞,等. 缺血性脑损伤中生物标志物的研究进展[J]. 国际麻醉学与复苏杂志,2022,43(10):1073-1078.
[4] 高永超,王爽,王静,等. 环状RNA在神经系统疾病中的研究进展[J]. 中国医药导报,2020,17(29):45-47.
[5] Zhang F, Zhang L, Zhao L, et al. The circular RNA Rap1b promotes Hoxa5 transcription by recruiting Kat7 and leading to increased Fam3a expression, which inhibits neuronal apoptosis in acute ischemic stroke[J]. Neural Regen Res, 2023,18(10):2237-2245.
[6] 黄英,陈兰,周英艳,等. CircRNA_ 0005853在老年急性脑梗死患者中的表达及其与认知功能障碍的相关性[J].中国医师杂志,2023,25(6):850-854.
[7] Liu Y, Li Y, Zang J, et al. CircOGDH is a penumbra biomarker and therapeutic target in acute ischemic stroke[J]. Circ Res,2022,130(6):907-924.
[8] Chen L. The expanding regulatory mechanisms and cellular functions of circular RNAs[J]. Nat Rev Mol Cell Biol, 2020,21(8):475-490.
[9] Wang X, Zhang S, Zhang Z, et al. Increased plasma levels of circPTP4A2 and circTLK 2 are associated with stroke injury[J]. Ann Clin Transl Neurol, 2023,10(8):1481-1492.
[10] Yang J, He W, Gu L, et al. CircFOXP1 alleviates brain injury after acute ischemic stroke by regulating STAT3/a poptotic signaling[J]. Transl Res,2023,257:15-29.
[11] Bai Y, Zhang Y, Han B, et al. Circular RNA DLGAP4 ameliorates ischemic stroke outcomes by targeting miR-143 to regulate endothelial-mesenchymal transition associated with blood-brain barrier integrity[J]. J Neurosci,2018,38(1):32-50.
[12] Cheng Q, Wang J, Li M, et al. CircSV2b participates in oxidative stress regulation through miR-5107-5 p-Foxk1-Akt1 axis in Parkinson's disease[J]. Redox Biol, 2022,56:102430.
[13] Xia X, Li X, Li F, et al. Correction to: a novel tumor suppressor protein encoded by circular AKT3 RNA inhibits glioblastoma tumorigenicity by competing with active phosphoinositide-dependent Kinase-1[J]. Mol Cancer, 2019,18(1):149.
[14] Bejot Y. Aging population and stroke: red flag[J]. Rev Neurol, 2020,176(10):751-753.
[15] Liu N, Jiang F, Chen Z, et al. circIFT80 functions as a ceRNA for miR-142, miR-568, and miR-634 and promotes the progression of colorectal cancer by targeting β-catenin[J]. Dis Markers, 2022,2022:8081246.
[16] Li P, Zhang Y, Liu H. The role of Wnt/β-catenin pathway in the protection process by dexmedetomidine against cerebral ischemia/reperfusion injury in rats[J]. Life Sci, 2019,236:116921.
[17] Song D, Zhang X, Chen J, et al. Wnt canonical pathway activator TWS119 drives microglial anti-inflammatory activation and facilitates neurological recovery following experimental stroke[J]. J Neuroinflammation, 2019,16(1):256.
[18] Librc R, Bramanti P, Mazzon E. The role of the Wnt canonical signaling in neurodegenerative diseases[J]. Life Sci, 2016,158:78-88.
