目的：观察槲皮素对胃食管反流大鼠食管黏膜屏障功能的影响,并探究其作用机制。方法：将40只SD大鼠随机分为空白对照组、模型组、槲皮素给药组、阳性对照组;除空白对照组外,其余建立反流性食管炎大鼠模型,槲皮素给药组给予槲皮素50 mg/kg,阳性对照组给予奥美拉唑1.52 mg/kg、铝碳酸镁56.89 mg/kg、吗丁啉1.14 mg/kg,空白对照组给予等容量生理盐水,每日一次,连续灌胃给药14天,末次给药后,大鼠麻醉后腹主动脉取血,处死,取食管组织样本,病理学染色,ELISA检测TNF-α、IL-6含量,WB检测IL-6、TNF-α表达量。结果：与空白对照组比较,模型组大鼠食管黏膜发生明显的病理变化,黏膜发红粗糙、缺损、鳞状上皮增生等;与模型组相比,槲皮素给药组的胃食管组织病理学变化减轻。与空白对照组比较,模型组IL-6、TNF-α含量明显升高(P＜0.05);与模型组比较,槲皮素给药组和阳性对照组IL-6、TNF-α含量明显降低(P＜0.05);WB结果显示,与模型组比较,槲皮素给药组与阳性对照组TNF-α、IL-6表达明显降低(P＜0.01)。结论：槲皮素可能通过减少炎症反应和促进胃食管黏膜修复来发挥其保护作用。
Objective: To observe the effect of quercetin on esophageal mucosal barrier function in rats with gastroesophageal reflux and explore its mechanism. Methods: Forty SD rats were randomly divided into blank control group, model group, quercetin administration group and positive control group. In addition to the blank control group, the rest of the rat model of reflux esophagitis was established. The quercetin administration group was given quercetin 50 mg/kg, the positive control group was given omeprazole 1.52 mg/kg, hydrotalcite 56.89 mg/kg, and domperidone 1.14 mg/kg. The blank control group was given an equal volume of normal saline once a day for 14 consecutive days. After the last administration, the rats were anesthetized and the blood was taken from the abdominal aorta, and the esophageal tissue samples were sacrificed. Then the pathological staining of esophageal tissue samples was performed, and the contents of TNF-α and IL-6 were detected by LISA, and the expression of IL-6 and TNF-α was detected by B. Results: Compared with the blank control group, the esophageal mucosa of the model group had obvious pathological changes, such as white and rough mucosa, defect, squamous epithelial hyperplasia, etc. Compared with the model group, the gastroesophageal histopathological changes in the quercetin treatment group were reduced. Compared with the blank control group, the contents of IL-6 and TNF-α in the model group were significantly increased (P＜0.05). Compared with the model group, the contents of IL-6 and TNF-α in the administration group and the positive control group were significantly decreased (P＜0.05). WB results showed that compared with the model group, the expression of TNF-α and IL-6 in the administration group and the positive control group was significantly decreased (P＜0.01). Conclusion: Quercetin may play a protective role by reducing inflammatory response and promoting gastroesophageal mucosal repair.
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