目的:探讨低氧预适应(hypoxic preconditioning,HPC)条件下对小鼠自然杀伤细胞(natural killer cell,NK)杀伤活性与功能的影响。方法:将小鼠分对照组(A组)、急性低氧组(B组)和HPC组(C组)3组,通过MTT比色法分别检测3组小鼠NK细胞的杀伤活性,通过EILSA检测NK细胞分泌干扰素-γ,Real-time PCR检测穿孔素、颗粒酶的表达量,并采用SPSS 19.0统计学软件进行数据分析。结果:低氧预适应组与急性低氧组相比NK杀伤力较强(P＜0.05),其中12 h时NK细胞活性明显增强(P＜0.05)。小鼠NK细胞分泌IFN-γ从4 h到16 h均逐渐下降(P＜0.05),显示低氧预适应组随着时间的延长较低氧组更能适应低氧;HPC组与B组相比低氧损伤减轻,分泌穿孔素、颗粒酶表达多于低氧组(P＜0.05)。结论:初步确定NK细胞在HPC与低氧组相比,NK细胞杀伤功能有所恢复;IFN-γ、穿孔素、颗粒酶蛋白的分泌量均有所升高。
Objective:To investigate the effect of hypoxic preconditioning (HPC) on NK cell killing activity in mice. Methods: Selected mice were divided into the control group (Group A), acute hypoxic group (Group B) and HPC group (Group C). The levels of interferon-γsecreted by NK cells were detected with EILSA, expression levels of perforin and granzyme were detected with Real-Time PCR, and the killing activities of NK cells of mice in the three groups were detected by MTT colorimetry. SPSS 19.0 statistical software was used for data analysis. Results: Compared with the group B, the killing activity of HPC group (Group C) was more powerful, and it obviously enhanced at 12 h(P＜0.05). The secretion of IFN-γ by NK cells was gradually decreased from 4 h to 16 h, which indicated that hypoxia preconditioning was more adaptive to hypoxia than the group B over time. Compared with the group B, the hypoxic injury of mice in the HPC group were relieved, and expression levels of perforin and granzyme were higher than that in the group B(P＜0.05). Conclusion: Compared with the acute hypoxic group, NK cell killing function in the HPC group was recovered, and secretions of IFN-γ, perforin and granzyme protein were increased.
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