目的: 探讨人参皂苷Rb1(GsRb1)对脂多糖(LPS)诱导急性肺损伤(ALI)的保护作用及其作用机制。方法: C57BL/6小鼠随机分为对照组、模型组、GsRb1给药组(低、中、高剂量组),气管内给予LPS造模,给药组在造模前3 d分别腹膜腔注射给予不同剂量GsRb1预处理,造模12 h将其麻醉,先采集肺泡灌洗液后再处死取出肺组织,计算肺湿/干比,试剂盒检测灌洗液白细胞介素-6(IL-6)、白细胞介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)水平以及肺髓过氧化物酶(MPO)活性,Western blot检测肺组织核因子-κB(NF-κB)蛋白的表达。结果: 高剂量GsRb1能明显降低肺组织湿/干比(P<0.05);中、高剂量GsRb1能明显降低肺组织MPO活性(P<0.05);高剂量GsRb1明显降低小鼠肺泡灌洗液IL-1β和TNF-α水平(P<0.05);高剂量GsRb1能明显降低肺组织NF-κB p65蛋白的表达(P<0.05)。结论: GsRb1可能通过调控NF-κB通路,减轻炎症反应,抑制LPS诱导的小鼠ALI。
Objective: To explore the protective effect and mechanism of ginsenoside Rb1 (GsRb1) on lipopolysaccharide (LPS)-induced acute lung injury (ALI). Methods: C57BL/6 mice were randomly divided into control group, model group, GsRb1 administration group (low, medium and high dose groups). LPS was given intratracheally to establish the model. The administration group was given intraperitoneal injection of different doses of GsRb1 pretreatment 3 days before modeling. After 12 hours of modeling, the alveolar lavage fluid was collected and the lung tissue was taken out. The lung wet/dry ratio was calculated. The levels of interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in lavage fluid and the activity of myeloperoxidase (MPO) in lung were detected by kit. The expression of nuclear factor-κB (NF-κB) protein in lung tissue was detected by Western blot. Results: High dose GsRb1 could significantly reduce the wet/dry ratio of lung tissue (P<0.05). Medium and high doses of GsRb1 could significantly reduce the MPO activity in lung tissue (P<0.05). High dose GsRb1 could significantly reduces the levels of IL-1β and TNF-α in alveolar lavage fluid of mice (P<0.05). High dose GsRb1 could significantly reduce the expression of NF-κB p65 protein in lung tissue (P<0.05). Conclusion: GsRb1 may reduce inflammation response and inhibit LPS-induced ALI in mice by regulating the NF-κB pathway.
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