目的: 利用网络药理学技术探讨中药人参皂苷Rb1治疗帕金森病的作用机制,为人参皂苷Rb1的临床合理应用提供科学理论依据。方法: 通过在SwissTargetPrediction、SEA以及SuperPred数据库中预测人参皂苷Rb1的靶点;使用关键词“Parkinson Disease”在GeneCards和Omim数据库中获得有关帕金森疾病的相关的靶点;将人参皂苷Rb1与帕金森病的靶点相互映射,作Veen图,从而获得交集基因;然后使用Cytoscape3.8.2软件构建“药物-作用靶点”网络;将韦恩图中与帕金森病相关的21个共有药物靶点导入String(https://string-db.org/)数据库中,进行蛋白-蛋白相互作用预测,从而预测出核心靶点。将人参皂苷Rb1治疗帕金森病的核心靶点导入到DAVID数据库中,得到GO分析结果和KEGG富集结果。利用 AutoDock Vina对人参皂苷Rb1和关键靶点进行分子对接,验证其相互作用活性。结果: 人参皂苷Rb1治疗帕金森病的核心靶点有4个,分别为BCL2L1、VEGFA、FGF2、KDR,相关通路28条。结合人参皂苷Rb1的生物学特性,发现人参皂苷Rb1治疗帕金森病可能是通过PI3K/Akt信号通路参与的生物调节过程,而此调节过程与细胞生物工程中的凋亡的相关机制密切相关。通过关键靶点与人参皂苷Rb1进行分子对接验证,靶点相互作用结合性高。结论: 人参皂苷Rb1治疗PD可能是通过减少细胞凋亡的生物过程发挥作用,影响的相关的通路有PI3K/Akt信号通路、MAPK信号通路、Notch信号通路,通过与BCL2L1、VEGFA、FGF2、KDR等靶点产生作用。
Objective: To explore the mechanism of ginsenoside Rb1 in the treatment of Parkinson 's disease by using network pharmacology technology, and to provide scientific theoretical basis for the clinical rational application of ginsenoside Rb1. Methods: The targets of ginsenoside Rb1 were predicted in SwissTargetPrediction, SEA and SuperPred databases. The related targets of Parkinson's disease were obtained in the GeneCards and Omim databases using the keyword 'Parkinson Disease'. The ginsenoside Rb1 and the target of Parkinson's disease were mapped to each other, and the Veen diagram was made to obtain the intersection gene. Then, Cytoscape 3.8.2 software was used to construct the 'drug-target' network ; the 21 common drug targets related to Parkinson's disease in the Venn diagram were imported into the String (https://string-db.org/) database for protein-protein interaction prediction, so as to predict the core targets. The core targets of ginsenoside Rb1 in the treatment of Parkinson's disease were imported into the DAVID database to obtain GO analysis results and KEGG enrichment results. AutoDock Vina was used to perform molecular docking between ginsenoside Rb1 and key targets to verify their interaction activity. Results: There were four core targets of ginsenoside Rb1 in the treatment of Parkinson's disease, namely BCL2L1, VEGFA, FGF2, KDR, with 28 related pathways. Combined with the biological characteristics of ginsenoside Rb1, it was found that ginsenoside Rb1 treatment of Parkinson's disease mght be involved in the biological regulation process through the PI3K/Akt signaling pathway, and this regulation process was closely related to the mechanism of apoptosis in cell bioengineering. Through the molecular docking verification of the key target and ginsenoside Rb1, the target interaction was highly combined. Conclusion: The mechanism of ginsenoside Rb1 in the treatment of PD may play a role by reducing the biological process of apoptosis. The related pathways affected are PI3K/Akt signaling pathway, MAPK signaling pathway, Notch signaling pathway, and BCL2L1, VEGFA, FGF2, KDR and other targets.
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