目的: 利用在线数据库和生物信息学的方法,研究BUB1B在胰腺腺癌(pancreatic adenocarcinoma,PAAD)组织中的表达水平及与预后和免疫细胞浸润的相关性。方法: 利用GEPIA数据库分析BUB1B在各种癌症中的表达情况;GEPIA、Kaplan-Meier Plotter数据库检索BUB1B的表达与PAAD患者预后的相关性;随后利用String数据库构建BUB1B共表达基因的蛋白互作网络,并用David数据库分析其GO生物学功能及KEGG信号通路;最后使用TIMER数据库分析BUB1B基因的表达与免疫浸润细胞之间的相关性。结果: 在PADD组织中,BUB1B表达水平高于正常组织(P＜0.01);BUB1B的高转录水平与性别、年龄、种族、肥胖状况、饮酒习惯、慢性胰腺炎、淋巴转移和癌症阶段密切相关(P＜0.05);BUB1B mRNA表达水平与PAAD预后和免疫细胞浸润相关(P＜0.05) 。结论: BUB1B在PADD中高表达,与PAAD预后、免疫浸润相关,可作为胰腺癌诊断、预后、药物治疗的潜在标志物。
Objective: To study the expression level, prognosis and correlation with immune cell infiltration of BUB1B in pancreatic adenocarcinoma ( PAAD ) by online database and bioinformatics. Methods: The expression of BUB1B in various cancers was analyzed by GEPIA database. The correlation between the expression of BUB1B and the prognosis of PAAD patients was searched by GEPIA and Kaplan-Meier Plotter database. Then, the protein interaction network of BUB1B co-expressed genes was constructed by using String database, and its GO biological function and KEGG signaling pathway were analyzed by using David database. Finally, the TIMER database was used to analyze the correlation between the expression of BUB1B gene and immune cell infiltrating cells. Results: The expression level of BUB1B in PADD tissues was higher than that in normal tissues (P＜0.01). The high transcription level of BUB1B was closely related to gender, age, race, obesity, drinking habits, chronic pancreatitis, lymph node metastasis and cancer stage (P＜0.05). The expression level of BUB1B mRNA was associated with prognosis and immune cell infiltration (P＜0.05). Conclusion: BUB1B is highly expressed in PADD, which is related to prognosis and immune infiltration, and can be used as a potential marker for diagnosis, prognosis and drug treatment of pancreatic cancer.
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