中国医学论著

香青兰提取物治疗溃疡性结肠炎的网络药理学分析*

  • 彭韵志 ,
  • 白文慧 ,
  • 陈张军 ,
  • 于慧 ,
  • 靳敏 ,
  • 胡海 ,
  • 王占黎
展开
  • 1.包头医学院研究生院,内蒙古包头 014040;
    2.包头医学院基础医学与法医学院;
    3.内蒙古自治区疾病相关生物标志物重点实验室

收稿日期: 2022-10-30

  网络出版日期: 2023-03-08

基金资助

*国家自然科学基金资助项目(No. 82060084);内蒙古自治区科技创新引导奖励资金项目

A network pharmacological analysis of Dracocephalum moldavica L. extract in the treatment of ulcerative colitis

  • PENG Yunzhi ,
  • BAI Wenhui ,
  • CHEN Zhangjun YU Hui ,
  • JIN Min ,
  • HU Hai ,
  • WANG Zhanli
Expand
  • 1. Graduate School, Baotou Medical College, Baotou 014040, China;
    2. School of Basic medicine, Baotou Medical College;
    3. Inner Mongolia Key Laboratory of Disease-Related Biomarkers, Baotou Medical College

Received date: 2022-10-30

  Online published: 2023-03-08

摘要

目的: 基于网络药理学探讨香青兰提取物治疗溃疡性结肠炎的作用机制。方法: 利用数据库收集香青兰提取物和溃疡性结肠炎的靶基因,通过蛋白质互作网络(Protein-Protein Interaction Networks,PPI)网络分析以及中心网络拓扑筛选,对靶基因进行基因本体论(GO)功能富集分析、《京都基因与基因组百科全书》(KEGG)通路富集分析。结果: 通过检索数据库共获得172个香青兰提取物活性成分治疗溃疡性结肠炎的潜在靶点,同时,利用PPI网络分析筛选出了关键治疗基因,并对蛋白质群进行了生物学功能注释,此外,KEGG通路富集分析表明香青兰提取物治疗溃疡性结肠炎时涉及消化、免疫系统反应和炎症反应的多条相关通路。结论: 香青兰提取物中的活性成分具有多种生物学功能,可作用于多条炎症、免疫及癌症相关通路,具有多成分、多靶点协同作用的特点。

本文引用格式

彭韵志 , 白文慧 , 陈张军 , 于慧 , 靳敏 , 胡海 , 王占黎 . 香青兰提取物治疗溃疡性结肠炎的网络药理学分析*[J]. 包头医学院学报, 2023 , 39(2) : 56 -61 . DOI: 10.16833/j.cnki.jbmc.2023.02.010

Abstract

Objective: To investigate the mechanism of action of Dracocephalum moldavica L. extract in the treatment of ulcerative colitis based on network pharmacology. Methods: The database was used to collect the target genes of Dracocephalum moldavica L. and ulcerative colitis. GO functional enrichment analysis and KEGG pathway enrichment analysis were performed on target genes through protein-protein interaction (PPI) network analysis and central topology screening. Results: A total of 172 potential targets for the treatment of ulcerative colitis with the active ingredients of Dracocephalum moldavica L. were obtained by searching the database. Meanwhile, the key therapeutic genes were screened by PPI network analysis, and the biological functions of the protein groups were analyzed for annotation. In addition, KEGG pathway enrichment analysis indicated multiple relevant pathways involved in digestion, immune system response and inflammatory response when Dracocephalum moldavica L. extract treating ulcerative colitis. Conclusions: The active ingredients in Dracocephalum moldavica L. extract have a variety of biological functions, which could act on sorts of inflammation, immune and cancer-related pathways for its multi-component and multi-target synergy.

参考文献

[1] Shanaida M, Jasicka-Misiak I, Makowicz E, et al. Development of high-performance thin layer chromatography method for identification of phenolic compounds and quantification of rosmarinic acid content in some species of the Lamiaceae family[J]. J Pharm Bioallied Sci, 2020, 12(2): 139-145.
[2] Wu C, Liu HZ, Rong XJ, et al. Phytochemical composition profile and space-time accumulation of secondary metabolites for dracocephalum moldavica Linn. via UPLC-Q/TOF-MS and HPLC-DAD method[J]. Biomed Chromatogr, 2020, 34(8): e4865.
[3] 天亮, 吴香杰, 梦月. 蒙药材香青兰的化学成分研究进展[J]. 世界最新医学信息文摘, 2017, 17(14): 31, 37.
[4] 何陈林, 孟和毕力格, 王秀兰, 等. 蒙药材香青兰的研究概况[J]. 中国民族医药杂志, 2018, 24(10): 35-38.
[5] 杨丽娜, 邢建国, 何承辉, 等. 维药香青兰的化学成分与药理作用评价[J]. 世界临床药物, 2013, 34(4): 226-231.
[6] 阿衣努尔·热合曼, 麦路德木·麦麦吐逊, 热西旦木·托乎提, 等. 香青兰化学成分分离纯化及结构鉴定[J]. 新疆医科大学学报, 2011, 34(4): 366-369.
[7] 戴晓庆, 汪豪, 叶文才, 等. 维药香青兰叶的化学成分研究[J]. 药学与临床研究, 2010, 18(3): 267-268, 273.
[8] 宋睿, 金传山, 周亚伟. 香青兰中总黄酮和单体的含量测定[J]. 中国实验方剂学杂志, 2010, 16(12): 71-74.
[9] Martínez-Vázquez M, Estrada-Reyes R, Martínez-Laurrabaquio A, et al. Neuropharmacological study of Dracocephalum moldavica L. (Lamiaceae) in mice: sedative effect and chemical analysis of an aqueous extract[J]. J Ethnopharmacol, 2012, 141(3): 908-917.
[10] Hu L, Wu C, Zhang ZJ, et al. Pinocembrin protects against dextran sulfate sodium-induced rats colitis by ameliorating inflammation, improving barrier function and modulating gut microbiota[J]. Front Physiol, 2019, 10: 908.
[11] Goh KI, Cusick ME, Valle D, et al. The human disease network[J]. Proc Natl Acad Sci USA, 2007, 104(21): 8685-8690.
[12] Zhang RZ, Zhu X, Bai H, et al. Network pharmacology databases for traditional Chinese medicine: review and assessment[J]. Front Pharmacol, 2019, 10: 123.
[13] Wu JW, Wei ZH, Cheng P, et al. Rhein modulates host purine metabolism in intestine through gut microbiota and ameliorates experimental colitis[J]. Theranostics, 2020, 10(23): 10665-10679.
[14] Szklarczyk D, Gable AL, Lyon D, et al. STRING v11:protein-protein association networks with increased coverage, supporting functional discovery in genome-wide experimental datasets[J]. Nucleic Acids Res, 2019, 47(D1): D607-D613.
[15] Jiang N, Li H, Sun YS, et al. Network pharmacology and pharmacological evaluation reveals the mechanism of the Sanguisorba officinalis in suppressing hepatocellular carcinoma[J]. Front Pharmacol, 2021, 12: 618522.
[16] Huang DW, Sherman BT, Lempicki RA. Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources[J]. Nat Protoc, 2009, 4(1): 44-57.
[17] Zhang C, Jiang M, Lu AP. Considerations of traditional Chinese medicine as adjunct therapy in the management of ulcerative colitis[J]. Clin Rev Allergy Immunol, 2013, 44(3): 274-283.
[18] Zheng K, Shen H, Jia J, et al. Traditional Chinese medicine combination therapy for patients with steroid-dependent ulcerative colitis: study protocol for a randomized controlled trial[J]. Trials, 2017, 18(1): 8.
[19] 郭子霞, 张丹参, 李炜. 大黄对溃疡性结肠炎治疗作用研究进展[J]. 中国药理学与毒理学杂志, 2021, 35(9): 655.
[20] Li BL, Du PL, Du Y, et al. Luteolin alleviates inflammation and modulates gut microbiota in ulcerative colitis rats[J]. Life Sci, 2021, 269: 119008.
[21] Sheng QS, Li F, Chen GP, et al. Ursolic acid regulates intestinal microbiota and inflammatory cell infiltration to prevent ulcerative colitis[J]. J Immunol Res, 2021, 2021: 6679316.
[22] Noviello D, Mager R, Roda G, et al. The IL23-IL17 immune axis in the treatment of ulcerative colitis: successes, defeats, and ongoing challenges[J]. Front Immunol, 2021, 12: 611256.
[23] Tatiya-Aphiradee N, Chatuphonprasert W, Jarukamjorn K. Immune response and inflammatory pathway of ulcerative colitis[J]. J Basic Clin Physiol Pharmacol, 2018, 30(1): 1-10.
[24] Yiu J HC, Dorweiler B, Woo CW. Interaction between gut microbiota and toll-like receptor: from immunity to metabolism[J]. J Mol Med, 2017, 95(1): 13-20.
[25] Price AE, Shamardani K, Lugo KA, et al. A map of toll-like receptor expression in the intestinal epithelium reveals distinct spatial, cell type-specific, and temporal patterns[J]. Immunity, 2018, 49(3): 560-575.
[26] Yan JB, Luo MM, Chen ZY, et al. The function and role of the Th17/treg cell balance in inflammatory bowel disease[J]. J Immunol Res, 2020, 2020: 8813558.
[27] Taylor CT, Colgan SP. Regulation of immunity and inflammation by hypoxia in immunological niches[J]. Nat Rev Immunol, 2017, 17(12): 774-785.
[28] Liu P, Lu ZW, Liu LL, et al. NOD-like receptor signaling in inflammation-associated cancers: from functions to targeted therapies[J]. Phytomedicine, 2019, 64: 152925.
[29] Platnich JM, Muruve DA. NOD-like receptors and inflammasomes: a review of their canonical and non-canonical signaling pathways[J]. Arch Biochem Biophys, 2019, 670: 4-14.
[30] Chen TL, Zhang XD, Zhu GL, et al. Quercetin inhibits TNF-α induced HUVECs apoptosis and inflammation via downregulating NF-kB and AP-1 signaling pathway in vitro[J]. Medicine (Baltimore), 2020, 99(38): e22241.
[31] Graves DT, Milovanova TN. Mucosal immunity and the FOXO1 transcription factors[J]. Front Immunol, 2019, 10: 2530.
[32] Wang YL, Graves DT. Keratinocyte function in normal and diabetic wounds and modulation by FOXO1[J]. J Diabetes Res, 2020, 2020: 3714704.
文章导航

/