目的: 采用纳米氧化铈(CeO2 NPs)建立大鼠肠炎模型,并确定肠炎的形成时间。方法: 将SD大鼠随机分为对照组和模型组,采用隔天灌胃的方式,对照组给予去离子水,模型组给予100 mg/kg CeO2 NPs。在每次给药15 d时,随机选取造模组3只大鼠取空肠、回肠、盲肠、结肠组织做HE染色进行病理形态学观察,确定肠炎形成时间。结果: 大鼠给药第45天,各肠道组织出现不同程度的炎症反应,提示造模成功。其中空肠和结肠炎症反应轻微,表现为局部炎症细胞浸润,细胞排列疏松。回肠和盲肠炎症反应明显,有大量炎症细胞浸润,腺体破坏,绒毛变短,提示造模成功。结论: 通过构建纳米氧化铈诱导下的大鼠肠炎模型确定了肠炎形成时间,为CeO2 NPs的毒理学研究提供实验依据。
Objective: the rat enteritis model was established by nano cerium dioxide (CeO2 NPs) and the formation time of enteritis was determined. Method: SD rats were randomly divided into the control group and the model group.The control group was given deionized water and the model group was given 100mg /kg CeO2 NPs by gavage every other day.Every 15 days after administration, three rats in the model group were randomly selected.The tissues of jejunum, ileum, cecum and colon were stained with H-E for pathomorphological observation to determine the formation time of enteritis. Result: On the 45th day after administration, the intestinal tissues of rats showed inflammatory reaction to various degrees, suggesting that the model was successful.The inflammatory reaction of jejunum and colon was mild, which showed local inflammatory cell infiltration and loose cell arrangement.The ileum and cecum had obvious inflammatory reaction; a large number of inflammatory cells infiltrate, glands were destroyed; villi became shorter, indicating the success of modeling. Conclusion: In this experiment, we constructed the rat enteritis model induced by nano ceria and determined the formation time of enteritis, so as to provide experimental basis for the toxicological study of CeO2 NPs.
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