目的: 基于16SrDNA行Illuminate测序技术研究包头地区老年特应性皮炎(AD)患者和健康老年人皮肤微生态的差异,为AD防治提供依据。方法: 收集2024年1月-2024年6月于包头医学院第一附属医院皮肤科就诊的老年特应性皮炎患者(PA组)15例及健康老年人(CK组)10例,PA取上肢皮损处皮肤微生态样本,CK组取相同部位皮肤样本,通过提取样本DNA进行Illuminate高通量测序。结果: (1)样本按97%的相似性进行聚类,共得到7 825条OTU序列,其中门、纲、目、科、属、种分别为59种,132种,261种,425种,1 053种,672种。(2)在门、纲、目、科、属、种分类层级中,PA组的厚壁菌门及其下属的孢杆菌纲、产球菌目、葡萄球菌科和葡萄球菌属的菌群丰度显著高于CK组(P＜0.05);变形菌门及其相关的变形菌纲、假单胞菌目、莫拉氏菌科和不动杆菌属的丰度则显著低于CK组(P＜0.05);在科水平上,两组间的物种丰度差异未达到统计学显著性(P＞0.05)。(3)LEfSe分析结果显示,厚壁菌门及其下属分类单元(包括芽孢杆菌纲、产球菌目、葡萄球菌科和葡萄球菌属)在两组菌群组成差异中起关键作用(P＜0.05)。(4)PA组与CK组间Alpha多样性分析差异无统计学意义(P＞0.05)。(5)Beta多样性分析表明PA组与CK组组间差异明显(P＜0.05)。结论: 老年AD患者与健康老年人皮肤菌群的组成及多样性存在明显差异,说明老年AD可能与皮肤微生态相关。
Objective: To study the difference of skin microecology between elderly patients with atopic dermatitis (AD) and healthy elderly people in Baotou area based on 16 SrDNA Illuminate sequencing technology, so as to provide basis for the prevention and treatment of AD. Methods: A total of 15 elderly patients with atopic dermatitis (PA group) and 10 healthy elderly people (CK group) were collected from the Department of Dermatology, the First Affiliated Hospital of Baotou Medical College from January 2024 to June 2024. PA collects skin microbiome samples from the affected area of the upper limb, and CK group samples were taken from the same part of the skin. Illuminate high-throughput sequencing was performed by extracting sample DNA. Results: (1) The samples were clustered according to 97% similarity, and a total of 7825 OTU sequences were obtained, of which the phylum, class, order, family, genus and species were 59, 132, 261, 425, 1053 and 672, respectively. (2) At the level of phylum, class, order, family, genus and species, the abundance of Firmicutes and its subordinate bacteroidetes, coccus, staphylococcus and staphylococcus in PA group was significantly higher than that in CK group (P＜0.05). The abundance of Proteobacteria and its related Proteobacteria, Pseudomonas, Moraxellaceae and Acinetobacter was significantly lower than that of CK group (P＜0.05). At the family level, there was no statistically significant difference in species abundance between the two groups (P＞0.05). (3) The results of LEfSe analysis showed that Firmicutes and its subordinate taxa ( including Bacillus, Coccobacteria, Staphylococci, and Staphylococcus ) played a key role in the difference in bacterial composition between the two groups (P＜0.05). (4) There was no significant difference in Alpha diversity between PA group and CK group (P＞0.05). (5) Beta diversity analysis showed that there was a significant difference between PA group and CK group (P＜0.05). Conclusion: There are significant differences in the composition and diversity of skin flora between elderly AD patients and healthy elderly people, indicating that elderly AD may be related to skin microecology.
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