目的：探讨半夏白术天麻汤(BBTD)经典复方对缺血性脑卒中的作用机制研究。方法：选用90只245~280 g SD大鼠,参考Zea-longa改良的线栓法制备MCAO动物模型,通过TTC染色检测MCAO模型动物的脑梗死面积,采用WB方法检测缺血性脑组织的缺血半暗带中IL-6的蛋白表达水平,并用RT-PCR方法检测缺血性脑组织的缺血半暗带中IL-6的mRNA表达水平。结果：模型组脑梗死面积百分比与假手术组相比较脑梗死面积百分比增加,差异具有统计学意义(P＜0.05);BBTD和尼莫地平药物处理组脑梗死面积百分比与模型组相比较,其脑梗死面积百分比均有不同程度的减少,差异具有统计学意义(P＜0.05)。模型组炎症因子IL-6的蛋白表达水平与假手术组相比较明显升高,差异具有统计学意义(P＜0.05);BBTD和尼莫地平药物处理组炎症因子IL-6的蛋白表达水平与模型组相比较,其IL-6的蛋白表达水平均有不同程度的降低,差异具有统计学意义(P＜0.05)。模型组炎症因子IL-6的 mRNA表达水平与假手术组相比较明显升高,差异具有统计学意义(P＜0.05);BBTD和尼莫地平药物处理组炎症因子IL-6的 mRNA表达水平与模型组相比较,其IL-6的 mRNA表达水平均有不同程度的降低,差异具有统计学意义(P＜0.05)。结论：经典复方半夏白术天麻汤可以有效缓解脑卒中事件中的缺血性脑损伤,这可能与IL-6所介导的炎症反应有关。
Abstract
Objective: To investigate the mechanism of Banxia Baizhu Tianma Decoction (BBTD) on ischemic stroke. Methods: A total of 90 SD rats (245-280 g) were selected to prepare MCAO animal model by referring to Zea-longa's modified suture method. The cerebral infarction area of MCAO model animals was detected by TTC staining. The protein expression level of IL-6 in ischemic penumbra of ischemic brain tissue was detected by WB method, and the mRNA expression level of IL-6 in ischemic penumbra of ischemic brain tissue was detected by RT-PCR method. Results: Compared with the sham operation group, the percentage of cerebral infarction area in the model group increased, and the difference was statistically significant (P＜0.05). Compared with the model group, the percentage of cerebral infarction area in BBTD and nimodipine treatment groups decreased to varying degrees, and the difference was statistically significant (P＜0.05). Compared with the sham operation group, the protein expression level of inflammatory factor IL-6 in the model group was significantly increased, and the difference was statistically significant (P＜0.05). Compared with the model group, the protein expression level of inflammatory factor IL-6 in the BBTD and nimodipine treatment groups was decreased to varying degrees, and the difference was statistically significant (P＜0.05). Compared with the sham operation group, the mRNA expression level of inflammatory factor IL-6 in the model group was significantly increased, and the difference was statistically significant (P＜0.05). Compared with the model group, the mRNA expression level of inflammatory factor IL-6 in BBTD and nimodipine treatment groups was decreased to varying degrees, and the difference was statistically significant (P＜0.05). Conclusion: Classic Banxia Baizhu Tianma Decoction can effectively alleviate ischemic brain injury in stroke events, which may be related to the inflammatory response mediated by IL-6.
关键词
半夏白术天麻汤 /
缺血性脑卒中 /
IL-6 /
脑梗死面积 /
蛋白表达 /
mRNA表达
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Key words
Banxia Baizhu Tianma Decoction /
Ischemic stroke /
IL-6 /
Cerebral infarction size /
Protein expression /
mRNA expression
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脚注
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基金
内蒙古自治区大学生创新项目(S202210130015);内蒙古自治区教育科学“十四五”规划课题(NGJGH2023520)
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