HUO Minfeng, YUE Juan, DONG Qiumei, LIU Xiping, LIU Jun, ZHANG Yuanyuan, LI Mingcheng
Objective: To study the mechanism of Banxia Xiexin decoction and its disassembled prescriptions on improving intestinal immunity of patients with inflammatory bowel disease (IBD). Methods: The active ingredients and targets of Banxia Xiexin decoction and its disassembled prescriptions were screened out from the Traditional Chinese Medicine System Pharmacology Database (TCMSP). Genecards and OMMI databases were used to screen potential targets related to inflammatory bowel disease. After intersection, Cytoscape 3.7.0 was used to make the active ingredient-target network chart, and the protein-protein interaction (PPI) network was drawn with String, and the signal pathway between drugs and inflammatory bowel disease was analyzed using GO and KEGG pathway enrichment analysis. Results: There were 129 active components in Banxia Xiexin decoction, 43 in Xinkai (Banxia + dried ginger) group, 104 in Kujiang (radix scutellariae + coptidis rhizoma) group and 121 in Ganbu (ginseng + radix glycyrrhizae + jujube) group. Further analysis found that Quercetin, Beta sitosterol, Kaemoferol, Stigmasterol, Baicalein and other active components in Banxia Xiexin Decoction group were closely related to PTGS1, AR, ESR1, PRSS1, PPARG and other IBD disease targets, and may be involved in the regulation through AGE-RAGE and TNF signaling pathway. In Xinkai group, the results showed that β-sitosterol, Baicalein, Stigmasterol, Cavendine), Coniferin and other active components were related to PTGS1, Chrm1, Chrm3, PGR, AR targets, and involved in P53 signaling pathway. Quercetin, Baicalein, Wogonin, Beta-sitosterol, Acacetin in Kujiang group were related to PTGS1, AR, PRSS1, ESR1 and CHEK1 targets, and involved in P13K-Akt, AGE-RAGE, P53 and TNF signaling pathway. In Ganbu group, Quercetin, Kaemoferol, β-sitosterol, Stigmasterol, Isorhamnetin and other active components were related to ESR1, PPARG, AR, PTGS1 and PRSS1 targets, and involved in AGE-RAGE and TNF signaling pathway by playing a role of anti-apoptosis, anti-inflammatory, and enhancing intestinal immunity to inhibit the occurrence and development of IBD. Conclusion: Banxia Xiexin decoction and its disassembled prescriptions could improve IBD for their multi-component, multi-target and multi pharmacological effects.
