Objective: To study the effect of cerium oxide nanoparticles on hippocampus of mice, and to explore whether cerium oxide nanoparticle could be used as the drug delivery carrier in the development and application of drugs for brain related diseases by analyzing the differential proteins in proteomics. Methods: ICR mice were randomly divided into the blank group and cerium oxide nanoparticles group. The cerium oxide nanoparticle group was injected with cerium oxide nanoparticles of 2-6 nm in particle size (0.4 mg/kg) through tail vein, and the control group was given the same amount of normal saline. Results: Compared with the control group, there was no significant difference in the behavior and histopathology of mice in the cerium oxide nanoparticles group. The proteomics analysis showed that 523 proteins were differentially expressed, including 243 down-regulated proteins and 280 up-regulated proteins. KEGG pathway analysis showed that the main pathways enriched in differential proteins included oxidative phosphorylation signaling pathway, Wnt signaling pathway, long-term enhancement signaling pathway, cholesterol metabolism pathway. Conclusion: Cerium oxide nanoparticles with a particle size of 2-6 nm and a dose of 0.4 mg/kg has no harmful effect on hippocampal tissue of mice and could be used as the carrier for drug delivery.
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