Objective: To observe the effects of Dracocephalum moldavica L. (DML) extract on the expression of AhR, I-κB and related inflammatory factors in rats with ulcerative colitis. Methods: Total 30 male SD rats were divided into three groups (n=10/group) randomly as follows: control group, model group and DML group. Experimental colitis was induced in rats by oral administration of 5 % DSS solution for 7 days. Beginning on Day 8, rats in DML group were given DML 400 mg/kg for 7 days. The control group and model group were given the same amount of physiological saline. The body weight, diarrhea degree and hematochezia were recorded daily. HE staining was used to observe the histopathological changes. The mRNA expression levels of AhR, IL-1β, IL-6 and IL-8 was detected by real time fluorescence quantitative PCR. The protein expression levels of p-I-κB in colon tissues were detected by Western blot. Results: Compared with control group, weight lost, had mucus, pus and blood in the stool, mucosal hemorrhage, inflammatory cell infiltration and submucosal edema were observed in model group. The expression levels of IL-1β, IL-6, IL-8, p-I-κB increased (P＜0.05), while the expression level of AhR decreased (P＜0.05). Compared with model group, the DAI score decreased,the pathological colon conditions were improved in DML group. The expression levels of IL-1β, IL-6, IL-8, p-I-κB decreased (P＜0.05), while the expression level of AhR increased (P＜0.05). Conclusion: Dracocephalum moldavica L. extract can regulate AhR and I-κB signaling pathways, inhibit the release of proinflammatory factors and reduce intestinal inflammatory response, so as to play a protective role in ulcerative colitis.
BAI Wenhui
,
PENG Yunzhi
,
HU Hai
,
JIN Min
,
CHEN Zhangjun
,
WANG Zhanli
,
YU Hui
. Effects of Dracocephalum moldavica L. extract on the expression of AhR/I-κB related pathway proteins and inflammatory factors in rats with ulcerative colitis[J]. Journal of Baotou Medical College, 2022
, 38(11)
: 1
-5
.
DOI: 10.16833/j.cnki.jbmc.2022.11.001
[1] Osada T, Ohkusa T, Okayasu I, et al. Correlations among total colonoscopic findings, clinical symptoms, and laboratory markers in ulcerative colitis [J]. J Gastroenterol Hepatol, 2008, 23 Suppl 2: S262-267.
[2] Li C, Wang J, Ma R, et al. Natural-derived alkaloids exhibit great potential in the treatment of ulcerative colitis [J]. Pharmacol Res, 2021: 105972.
[3] Yu M, Wang Q, Ma Y, et al. Aryl hydrocarbon receptor activation modulates intestinal epithelial barrier function by maintaining tight junction integrity [J]. Int J Biol Sci, 2018, 14(1): 69-77.
[4] Pernomian L, Duarte-silva M, De barros cardoso CR. The Aryl hydrocarbon receptor (AHR) as a potential target for the control of intestinal inflammation: insights from an immune and bacteria sensor receptor [J]. Clin Rev Allergy Immunol, 2020, 59(3): 382-390.
[5] Shimizu T, Takagi C, Sawano T, et al. Indigo enhances wound healing activity of Caco-2 cells via activation of the aryl hydrocarbon receptor [J]. J Nat Med, 2021, 75(4): 833-839.
[6] 张华然. 香青兰的化学成分及生物活性研究 [D]. 济南: 山东大学, 2019.
[7] 高洪锋, 陈爱春, 查淑娟, 等. 白黎芦醇和香青兰总黄酮抑制哮喘小鼠TNF-α和IL-6的释放 [J]. 基因组学与应用生物学, 2020, 39(3): 1295-1300.
[8] 热则耶·麦麦提敏. 香青兰总黄酮对H2O2诱导的人脐静脉内皮细胞氧化损伤的保护作用研究 [D].乌鲁木齐: 新疆医科大学, 2021.
[9] Hiraide T, Teratani T, Uemura S, et al. Pulmonary arterial hypertension caused by AhR signal activation protecting against colitis [J]. Am J Respir Crit Care Med, 2021, 203(3): 385-358.
[10] Lv Q, Shi C, Qiao S, et al. Alpinetin exerts anti-colitis efficacy by activating AhR, regulating miR-302/DNMT-1/CREB signals, and therefore promoting Treg differentiation [J]. Cell Death Dis, 2018, 9(9): 890.
[11] 刘云, 刘敏, 于慧, 等. 香青兰提取物对临床来源病原菌的抑制活性及生物信息学研究 [J]. 中国药房, 2020, 31(6): 666-670.
[12] 李梁, 蔡志平, 白雪梅, 等. 香青兰总黄酮促进脑缺血再灌注大鼠室管膜下区巢蛋白表达 [J]. 神经解剖学杂志, 2020, 36(6): 633-640.
[13] 赵云丽, 袁勇, 马晓莉, 等. 基于AMPK/SIRT1/PGC-1α信号通路研究香青兰总黄酮对大鼠心肌缺血再灌注损伤的保护机制 [J]. 中国药房, 2021, 32(3): 278-283.
[14] 周联, 李雁扬, 罗霞. 芳香烃受体在肠道黏膜免疫中的功能以及中药干预进展 [J]. 中国中西医结合杂志, 2021, 41(8): 1017-1023.
[15] Metidji A, Omenetti S, Crotta S, et al. The environmental sensor AHR protects from inflammatory damage by maintaining intestinal stem cell homeostasis and barrier integrity [J]. Immunity, 2018, 49(2): 353-362.e5.
[16] Peng C, Wu C, Xu X, et al. Indole-3-carbinol ameliorates necroptosis and inflammation of intestinal epithelial cells in mice with ulcerative colitis by activating aryl hydrocarbon receptor [J]. Exp Cell Res, 2021, 404(2): 112638.
[17] 蔺晓源, 刘杰民. TLR4/MyD88/NF-κB信号通路与溃疡性结肠炎 [J]. 胃肠病学, 2013, 18(4): 244-246.
[18] Yu ZH, Huang F, Xu N, et al. Expression of Toll-like receptor 4, CD14, and NF-κB in Chinese patients with ulcerative colitis [J]. J Immunoassay Immunochem, 2011, 32(1): 47-56.
[19] Soni JM, Sardoiwala MN, Choudhury SR, et al. Melatonin-loaded chitosan nanoparticles endows nitric oxide synthase 2 mediated anti-inflammatory activity in inflammatory bowel disease model [J]. Mater Sci Eng C Mater Biol Appl, 2021, 124: 112038.
[20] Zhou M, Xu W, Wang J, et al. Boosting mTOR-dependent autophagy via upstream TLR4-MyD88-MAPK signalling and downstream NF-κB pathway quenches intestinal inflammation and oxidative stress injury [J]. E Bio Medicine, 2018, 35: 345-360.
[21] Zhao C, Hu X, Bao L, et al. Aryl hydrocarbon receptor activation by Lactobacillus reuteri tryptophan metabolism alleviates Escherichia coli-induced mastitis in mice [J]. PLoS Pathog, 2021, 17(7): e1009774.
[22] Salisbury RL, Sulentic CE. The AhR and NF-κB/Rel proteins mediate the inhibitory effect of 2,3,7,8-Tetrachlorodibenzo-p-Dioxin on the 3′ immunoglobulin heavy chain regulatory region [J]. Toxicol Sci, 2015, 148(2): 443-459.
